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Titolo:
Direct and LC analysis of five benzodiazepines in human urine plasma usingan ADS restricted access extraction column
Autore:
Mullett, WM; Pawliszyn, J;
Indirizzi:
Univ Waterloo, Dept Chem, Waterloo, ON N2L 3G1, Canada Univ Waterloo Waterloo ON Canada N2L 3G1 em, Waterloo, ON N2L 3G1, Canada
Titolo Testata:
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
fascicolo: 5-6, volume: 26, anno: 2001,
pagine: 899 - 908
SICI:
0731-7085(200112)26:5-6<899:DALAOF>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; SOLID-PHASE EXTRACTION; PRECOLUMN PACKINGS; GAS-CHROMATOGRAPHY; MICRO EXTRACTION; WHOLE-BLOOD; SERUM; FLUNITRAZEPAM; METABOLITES; DIAZEPAM;
Keywords:
benzodiazepine analysis; ADS extraction precolumn; direct injection; reverse-phase chromatography; serum; urine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Pawliszyn, J Univ Waterloo, Dept Chem, Waterloo, ON N2L 3G1, Canada Univ Waterloo Waterloo ON Canada N2L 3G1 ON N2L 3G1, Canada
Citazione:
W.M. Mullett e J. Pawliszyn, "Direct and LC analysis of five benzodiazepines in human urine plasma usingan ADS restricted access extraction column", J PHARM B, 26(5-6), 2001, pp. 899-908

Abstract

An alkyl-diol-silica (ADS) precolumn was used for the direct and on-line extraction of several benzodiazepines from serum and urine. The protein component of the biological sample was flushed through the ADS column, while simultaneously extracting the benzodiazepine compounds in the pores of the ADS stationary phase, The role of hydrophobic interactions in the extraction mechanism was confirmed. Column switching was employed to elute the extracted analytes from the ADS column into a high-performance liquid chromatography reverse-phase C18 column for the isocratic separation and UV detection of the benzodiazepines. Sample preconcentration via large volume injections was possible, improving the limits of detection. The calculated clonazepam,oxazepam, temazepam, nordazepam and diazepam detection limits were 38.8, 24.2, 31.7, 31.3, 45.0 ng/ml in serum, respectively, and 48.4, 24.5, 31.7, 33.1, 52.9 ng/ml for urine, respectively. The method was linear over the range of 50-10000 ng/ml in both matrices with an average linear coefficient (R-2) value of 0.9918. The injection repeatability and intra-assay precision of the method were evaluated over ten injections, resulting in a percent relative standard deviation < 5%. The ADS extraction column was robust, providing many direct injections of biological fluids for the extraction and subsequent determination of benzodiazepines. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 11:25:15