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Titolo:
Analysis of intracellular didanosine triphosphate at sub-ppb level using LC-MS/MS
Autore:
Cahours, X; Tran, TT; Mesplet, N; Kieda, C; Morin, P; Agrofoglio, LA;
Indirizzi:
Univ Orleans, CNRS, Inst Chim Organ & Analyt, F-45067 Orleans, France UnivOrleans Orleans France F-45067 an & Analyt, F-45067 Orleans, France CNRS, UPR, Ctr Biophys Mol, F-45067 Orleans, France CNRS Orleans France F-45067 PR, Ctr Biophys Mol, F-45067 Orleans, France
Titolo Testata:
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
fascicolo: 5-6, volume: 26, anno: 2001,
pagine: 819 - 827
SICI:
0731-7085(200112)26:5-6<819:AOIDTA>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
TANDEM MASS-SPECTROMETRY; BLOOD MONONUCLEAR-CELLS; REVERSE-TRANSCRIPTASE; LIQUID-CHROMATOGRAPHY; RADIOIMMUNOASSAY;
Keywords:
didanosine; didanosine triphosphate; HPLC; MS/MS; HIV; electrospray; CEM-T4 cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Agrofoglio, LA Univ Orleans, CNRS, Inst Chim Organ & Analyt, BP 6759, F-45067 Orleans, France Univ Orleans BP 6759 Orleans France F-45067 rleans, France
Citazione:
X. Cahours et al., "Analysis of intracellular didanosine triphosphate at sub-ppb level using LC-MS/MS", J PHARM B, 26(5-6), 2001, pp. 819-827

Abstract

An analytical procedure has been developed for the analysis of intracellular didanosine triphosphate (ddATP). An electrospray ionization tandem mass spectrometer (ESI-MS) was interfaced to liquid chromatography (LC) using a mobile phase CH3OH/H2O (25/75) containing 1% formic acid for the analysis of the 5'-triphosphate metabolite of the antiviral didanosine. In this procedure. ddATP was extracted from CEM-T4 cells, isolated using an exchange anion solid phase extraction procedure, enzymatically dephosphorylated and then analyzed by LC-MS/MS within a I min run time. The influence of several parameters (electrospray ionization interface, acidic modifiers of the mobilephase) has been studied. A calibration curve was generated and the linear regression analysis yielded a regression coefficient (r(2)) greater than 0.999. Using LC-MS/MS detection in single reaction monitoring mode (SRM), thelimit of quantitation of ddA in CEM-T4 cells was 0.02 ng ml(-1). Furthermore, this procedure could be used to perform simultaneous detection of five nucleoside reverse transcriptase inhibitors, such as AZT, 3TC. ddA, ddC andd4T and make LC-MS/MS a method of choice for Therapeutic Drug Monitoring (TDM) in a clinical environment. (C) 2001 Elsevier Science B.V. All rights reserved.

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Documento generato il 06/04/20 alle ore 08:37:26