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Titolo:
Novel method for localized, functional sympathetic nervous system denervation of peripheral tissue using guanethidine
Autore:
Demas, GE; Bartness, TJ;
Indirizzi:
Georgia State Univ, Dept Biol, Ctr Behav Neurosci, Atlanta, GA 30303 USA Georgia State Univ Atlanta GA USA 30303 v Neurosci, Atlanta, GA 30303 USA Georgia State Univ, Dept Psychol, Atlanta, GA 30303 USA Georgia State Univ Atlanta GA USA 30303 pt Psychol, Atlanta, GA 30303 USA
Titolo Testata:
JOURNAL OF NEUROSCIENCE METHODS
fascicolo: 1, volume: 112, anno: 2001,
pagine: 21 - 28
SICI:
0165-0270(20011115)112:1<21:NMFLFS>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
WHITE ADIPOSE-TISSUE; ADULT-RATS; PROLIFERATION; METABOLISM; NERVES;
Keywords:
saporin; sympathectomy; adipose tissue; lipolysis; spleen; Siberian hamsters axotomy; norepinephrine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Bartness, TJ Georgia State Univ, Dept Biol, Ctr Behav Neurosci, 24 Peachtree Ctr Ave, Atlanta, GA 30303 USA Georgia State Univ 24 Peachtree Ctr Ave Atlanta GA USA 30303
Citazione:
G.E. Demas e T.J. Bartness, "Novel method for localized, functional sympathetic nervous system denervation of peripheral tissue using guanethidine", J NEUROSC M, 112(1), 2001, pp. 21-28

Abstract

A simple technique for local chemical sympathectomy of peripheral tissues is described using guanethidine. Multiple microinjections of guanethidine were made into inguinal or epididymal white adipose tissue (IWAT and EWAT) pads or spleens of hamsters. Guanethidine virtually abolished the sympathetic innervation of both EWAT and IWAT, as measured by the absence of significant norepinephrine (NE) tissue content two weeks later and as suggested by the two-fold increase in IWAT mass characteristic of surgically induced WATdenervation. These measures were not affected in the contralateral pads given equivolumetric injections of saline. Guanethidine injections into the spleen lead to a functional sympathectomy, as indicated by significant depletions of NE content. Because guanethidine treatment did not decrease body mass, induce ptosis, or spread to closely associated adjacent tissue (contralateral EWAT pad), no chemical-induced malaise or global sympathetic denervation was suggested. Guanethidine was more effective than two other local sympathectomy treatments, injections of the sympathetic neurotoxin anti-dopamine-beta -hydroxylase saporin or surgical denervation, in decreasing IWAT NE content and increasing IWAT pad mass. Collectively, these results suggest that locally applied, chemical sympathectomy with guanethidine provides an effective, restricted method for sympathectomizing WAT, spleen and likelyother peripheral tissues. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/21 alle ore 04:46:17