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Titolo:
Regional differences in neurotrophin availability regulate selective expression of VGF in the developing limbic cortex
Autore:
Eagleson, KL; Fairfull, LD; Salton, SRJ; Levitt, P;
Indirizzi:
Univ Pittsburgh, Sch Med, Dept Neurobiol, Pittsburgh, PA 15261 USA Univ Pittsburgh Pittsburgh PA USA 15261 urobiol, Pittsburgh, PA 15261 USA CUNY Mt Sinai Sch Med, Fishberg Res Ctr Neurobiol, New York, NY 10029 USA CUNY Mt Sinai Sch Med New York NY USA 10029 obiol, New York, NY 10029 USA
Titolo Testata:
JOURNAL OF NEUROSCIENCE
fascicolo: 23, volume: 21, anno: 2001,
pagine: 9315 - 9324
SICI:
0270-6474(200112)21:23<9315:RDINAR>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
NERVE GROWTH-FACTOR; CEREBRAL CORTICAL PROGENITORS; ANTEROGRADE AXONAL-TRANSPORT; ACTIVITY-DEPENDENT RELEASE; MESSENGER-RNA EXPRESSION; HIPPOCAMPAL-NEURONS; ADULT-RAT; GENE-EXPRESSION; DEVELOPMENTAL EXPRESSION; STORAGE COMPARTMENTS;
Keywords:
BDNF; NT-3; neurotrophins; VGF; cortical development; ELISA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
88
Recensione:
Indirizzi per estratti:
Indirizzo: Eagleson, KL Univ Pittsburgh, Sch Med, Dept Neurobiol, 3550 Terrace Ave, Pittsburgh, PA15213 USA Univ Pittsburgh 3550 Terrace Ave Pittsburgh PA USA 15213 USA
Citazione:
K.L. Eagleson et al., "Regional differences in neurotrophin availability regulate selective expression of VGF in the developing limbic cortex", J NEUROSC, 21(23), 2001, pp. 9315-9324

Abstract

Gene and protein expression patterns in the cerebral cortex are complex and often change spatially and temporally through development. The signals that regulate these patterns are primarily unknown. In the present study, we focus on the regulation of VGF expression, which is limited to limbic cortical areas early in development but later expands into sensory and motor areas. We isolated neurons from embryonic day 17 rat cortex and demonstrate that the profile of VGF expression in perirhinal (expressing) and occipital (nonexpressing) populations in vitro is similar to that in the perinatal cortex in vivo. The addition of neutralizing neurotrophin antibodies indicatesthat endogenous brain-derived neurotrophic factor (BDNF) is necessary for the normal complement of VGF-expressing neurons in the perirhinal cortex, although endogenous neurotrophin-3 (NT-3) regulates the expression of VGF ina subpopulation of cells. ELISA analysis demonstrates that there is significantly more BDNF present in the perirhinal cortex compared with the occipital cortex in the perinatal period. However, the total amount of NT-3 is similar between the two regions and, moreover, there is considerably more NT-3 than BDNF in both areas, a finding seemingly in conflict with regional VGF expression. Quantification of the extracellular levels of neurotrophins in perirhinal and occipital cultures using ELISA in situ analysis indicates that perirhinal neurons release significantly more BDNF than the occipital population. Furthermore, the amount of NT-3 released by the perirhinal neurons is significantly less than the amount of BDNF. Local injection of BDNF in vivo into a normally negative VGF region results in robust ectopic expression of VGF. These data suggest that the local availability of specific neurotrophins for receptor occupation, rather than the total amount of neurotrophin, is a critical parameter in determining the selective expression of VGF in the developing limbic cortex.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 06:45:07