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Titolo:
Analysis of albumins, using albumin blue 580, by capillary electrophoresisand laser-induced fluorescence
Autore:
Tseng, WL; Chiu, TC; Weng, JM; Chang, HT;
Indirizzi:
Natl Taiwan Univ, Dept Chem, Taipei 10764, Taiwan Natl Taiwan Univ Taipei Taiwan 10764 iv, Dept Chem, Taipei 10764, Taiwan
Titolo Testata:
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
fascicolo: 19, volume: 24, anno: 2001,
pagine: 2971 - 2982
SICI:
1082-6076(2001)24:19<2971:AOAUAB>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN SERUM-ALBUMIN; LIQUID-CHROMATOGRAPHY; ZONE ELECTROPHORESIS; NATIVE PROTEINS; SEPARATION; ASSAY; PEPTIDES; SULFATE; IDENTIFICATION; ANTIBODY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Chang, HT Natl Taiwan Univ, Dept Chem, Taipei 10764, Taiwan Natl Taiwan Univ Taipei Taiwan 10764 hem, Taipei 10764, Taiwan
Citazione:
W.L. Tseng et al., "Analysis of albumins, using albumin blue 580, by capillary electrophoresisand laser-induced fluorescence", J LIQ CHR R, 24(19), 2001, pp. 2971-2982

Abstract

We report a new method for analysis of albumins with albumin blue 580 (AB 580) by capillary electrophoresis (CE) using a relatively low-cost He-Ne laser. During separation, albumins and AB 580 formed complexes bearing intensive fluorescence in Trisborate (TB) buffers. The analysis of human serum albumin (HSA) was fast (4 min); reproducible (relative standard deviation (RSD) values of the migration time and peak height were less than 1% and 2%, respectively); and sensitive (the limit of detection at signal-to-noise ratio = 3 was 11 DM). Compared to other common dyes, such as bromphenol blue and merocyanine 540 for detecting albumins, AB 580 provides advantages of lowfluorescence background, stability, sensitivity and selectivity. Without sample pretreatment, this proposed method was employed to determine HSA in urine and blood cells from a normal male, with results of 5.2 +/- 0.2 mg/L and about 8.2 +/- 0.2 zmol/cell. To further increase the resolvingpower of this method, the tryptic digest of HSA was separated using 0.6% PEO. From the fact that only two peaks were shown in the electropherogram, we suggested that HSA and AB 580 formed 1:1 complexes. This method also allowed for rapid separation of HSA, deoxyribonuclease I, and their complexes.

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Documento generato il 10/04/20 alle ore 15:54:13