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Titolo:
Interactions between dendritic cells and cytokine-induced killer cells lead to an activation of both populations
Autore:
Marten, A; Ziske, C; Schottker, B; Renoth, S; Weineck, S; Buttgereit, P; Schakowski, F; von Rucker, A; Sauerbruch, T; Schmidt-Wolf, IGH;
Indirizzi:
Univ Bonn, Med Klin & Poliklin 1, D-53105 Bonn, Germany Univ Bonn Bonn Germany D-53105 Klin & Poliklin 1, D-53105 Bonn, Germany Univ Bonn, Inst Klin Biochem, D-53105 Bonn, Germany Univ Bonn Bonn Germany D-53105 Inst Klin Biochem, D-53105 Bonn, Germany
Titolo Testata:
JOURNAL OF IMMUNOTHERAPY
fascicolo: 6, volume: 24, anno: 2001,
pagine: 502 - 510
SICI:
1524-9557(200111/12)24:6<502:IBDCAC>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNOLOGICAL EFFECTOR-CELLS; NK T-CELLS; IN-VIVO; ANTITUMOR-ACTIVITY; DIFFERENTIATION; EXPRESSION; IMMUNITY; MODEL; INTERLEUKIN-7; LYMPHOCYTES;
Keywords:
dendritic cells; cytokine-induced killer cells; costimulatory molecules; MHC class 2; natural killer T cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Schmidt-Wolf, IGH Univ Bonn, Med Klin & Poliklin 1, Sigmund Freud Str 25, D-53105 Bonn, Germany Univ Bonn Sigmund Freud Str 25 Bonn Germany D-53105 any
Citazione:
A. Marten et al., "Interactions between dendritic cells and cytokine-induced killer cells lead to an activation of both populations", J IMMUNOTH, 24(6), 2001, pp. 502-510

Abstract

Dendritic cells (DCs) are major antigen-presenting cells, They are capableof capturing and processing tumor antigens, expressing lymphocyte costimulatory molecules, and secreting cytokines to initiate immune responses. Here, the authors tested the effect of cytokine-induced killer (CIK) cells, a population that includes CD3(+)CD56(+) cells (natural killer T cells), with regard to their capacity to immuno-modulate DCs. Cytokine-induced killer cells were cocultured with autologous DCs Generated from peripheral blood mononuclear cells. Expression of markets typical for both populations was measured using flow cytometry, and secretion of interleukin (IL)-12 was determined using enzyme-linked immunosorbent assays. Cytotoxicity assays were performed to investigate the role of IL-12 and the importance of cell-cell interactions. Considering this, receptors for IL-12 and CD40 were blocked and cocultures were performed with cell culture inserts. Coculture of CIK cells led to a significant increase of DC-specific, costimulatory, and antigen-presenting molecules in DC cultures. In addition, coculture resulted in a dramatically increase of IL-12 secretion by DCs and to a significant increase in cytotoxic activity of CIK cells toward carcinoma cells. Blockage of IL-12 uptake decreased the cytolytic activity of CIK cells. Cytokine secretion was shown to be important for activation of CIK cells, and also cellular interactions between DCs and effector cells caused a higher cytolytic capacity. Interactions between DCs and CIK cells caused changes in the surface molecule expression of both populations, led to an increase of IL-12 secretion, and rendered an improved cytotoxic activity. The natural killer T cell subpopulation seems to be responsible for this effect. Therefore, coculture of DCs with CIK cells may have a major impact on immunotherapeutic protocolsfor patients with cancer.

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Documento generato il 29/03/20 alle ore 09:00:18