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Titolo:
Antitumor reactivity of lymph node cells primed in vivo with dendritic cell-based vaccines
Autore:
Tanigawa, K; Takeshita, N; Eickhoff, GA; Shimizu, K; Chang, AE;
Indirizzi:
Univ Michigan, Ctr Comprehens Canc, Div Surg Oncol, Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 Surg Oncol, Ann Arbor, MI 48109 USA
Titolo Testata:
JOURNAL OF IMMUNOTHERAPY
fascicolo: 6, volume: 24, anno: 2001,
pagine: 493 - 501
SICI:
1524-9557(200111/12)24:6<493:AROLNC>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-INFILTRATING LYMPHOCYTES; ADOPTIVE IMMUNOTHERAPY; MELANOMA PATIENTS; NAIVE PRECURSORS; ANTIGEN; INTERLEUKIN-2; VACCINATION; GENERATION; ANTI-CD3; CANCER;
Keywords:
dendritic cell; adoptive immunotherapy; vaccine; keyhole limpet hemocyanin (KLH); T cell;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Chang, AE 3302 Canc Ctr, 1500 E Med Ctr Dr, Ann Arbor, MI 48109 USA 3302 Canc Ctr 1500 E Med Ctr Dr Ann Arbor MI USA 48109 48109 USA
Citazione:
K. Tanigawa et al., "Antitumor reactivity of lymph node cells primed in vivo with dendritic cell-based vaccines", J IMMUNOTH, 24(6), 2001, pp. 493-501

Abstract

Tumor lysate-pulsed dendritic cells were used to generate nodal effector Tcells in the murine MCA 205 tumor model. Dendritic cells were derived frombone marrow and cultured in granulocyte-macrophage colony-stimulating factor/interleukin 4 before pulsation with tumor lysate. Multiple subcutaneous administrations of tumor lysate-pulsed dendritic cells (TP-DCs) resulted inan approximately eightfold hypertrophy of the vaccine draining nodes, withan increased influx of dendritic (CD11c(+)/CD80(+)) cells and B (B220(+)) cells. The vaccine-primed lymph node (VPLN) cells were secondarily activated with anti-CD3/interleukin 2 and exhibited specific interferon-gamma release to tumor antigen. The adoptive transfer of TP-DC VPLN cells resulted in regression of established 3-day pulmonary metastases. The antitumor reactivity of TP-DC VPLN cells was comparable to anti-CD3/interleukin 2 activated tumor-draining lymph node cells. However, the admixture of keyhole limpet hemocyanin (KLH) with tumor lysate during pulsation of dendritic cells significantly enhanced the induction of tumor-reactive VPLN cells. Tumor lysate-pulsed dendritic cells can be used as a strategy to generate effector T cells for adoptive immunotherapy.

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Documento generato il 05/04/20 alle ore 21:44:22