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Titolo:
Neutrophil proteinase 3-mediated induction of bioactive IL-18 secretion byhuman oral epithelial cells
Autore:
Sugawara, S; Uehara, A; Nochi, T; Yamaguchi, T; Ueda, H; Sugiyama, A; Hanzawa, K; Kumagai, K; Okamura, H; Takada, H;
Indirizzi:
Tohoku Univ, Sch Dent, Dept Microbiol & Immunol, Aoba Ku, Sendai, Miyagi 9808575, Japan Tohoku Univ Sendai Miyagi Japan 9808575 Ku, Sendai, Miyagi 9808575, Japan Tohoku Univ, Dept Anim Prod Sci, Grad Sch Agr Sci, Sendai, Miyagi 980, Japan Tohoku Univ Sendai Miyagi Japan 980 ch Agr Sci, Sendai, Miyagi 980, Japan Hyogo Med Univ, Host Def Lab, Inst Adv Med Sci, Nishinomiya, Hyogo, Japan Hyogo Med Univ Nishinomiya Hyogo Japan ed Sci, Nishinomiya, Hyogo, Japan Seiryo Dent Clin, Sendai, Miyagi, Japan Seiryo Dent Clin Sendai Miyagi Japan yo Dent Clin, Sendai, Miyagi, Japan Tcell Res Inst, Sendai, Miyagi, Japan Tcell Res Inst Sendai Miyagi JapanTcell Res Inst, Sendai, Miyagi, Japan
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 11, volume: 167, anno: 2001,
pagine: 6568 - 6575
SICI:
0022-1767(200112)167:11<6568:NP3IOB>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAMMA-INDUCING FACTOR; HUMAN GINGIVAL FIBROBLASTS; ENDOTHELIAL-CELLS; INTERFERON-GAMMA; T-CELLS; WEGENERS GRANULOMATOSIS; FUNCTIONAL-PROPERTIES; HUMAN INTERLEUKIN-18; CONVERTING-ENZYME; SERINE PROTEASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Sugawara, S Tohoku Univ, Sch Dent, Dept Microbiol & Immunol, Aoba Ku, 4-1 SeiryoMachi,Sendai, Miyagi 9808575, Japan Tohoku Univ 4-1 SeiryoMachi Sendai Miyagi Japan 9808575 Japan
Citazione:
S. Sugawara et al., "Neutrophil proteinase 3-mediated induction of bioactive IL-18 secretion byhuman oral epithelial cells", J IMMUNOL, 167(11), 2001, pp. 6568-6575

Abstract

IL-18, a potent IFN-gamma -inducing cytokine, is expressed by various nonimmune cells as well as macrophages, suggesting that it has important physiological and immunological roles. The present study focused on the mechanismof active IL-18 induction from human oral epithelial cells. The epithelialcells and the cell lines constitutively express IL-18 mRNA and the 24-kDa precursor form of IL-18. Bioactive IL-18 exhibiting IFN-gamma -inducing activity was detected in the supernatant of the cells on costimulation with neutrophil proteinase 3 (PR3) and LPS for 24 h after IFN-gamma -priming for 3days. An active 18-kDa form of IL-18 was detected in lysate and supernatant of the cells only after the above treatment and the induction was inhibited by cycloheximide and by serine proteinase inhibitors. After the treatment, lactate dehydrogenase activity was not detected in the cell culture supernatant, and PR3 was detected only in the membrane and not in cytoplasm fractions of the cells. Caspase-1 was not detected in the cells even after thetreatment and the IL-18 induction was not inhibited by a caspase-1 inhibitor. These results suggest that the PR3-mediated induction of bioactive IL-18 secretion from oral epithelial cells in combination with LPS after IFN-gamma -priming occurred via a caspase-1-independent pathway, and provide new insight into the possible involvement of a neutrophil proteinase in the induction of bioactive IL-18 in oral inflammation such as periodontitis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 18:44:23