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Titolo:
A novel mimetic antigen eliciting protective antibody to Neisseria meningitidis
Autore:
Granoff, DM; Moe, GR; Giuliani, MA; Adu-Bobie, J; Santini, L; Brunelli, B; Piccinetti, F; Zuno-Mitchell, P; Lee, SS; Neri, P; Bracci, L; Lozzi, L; Rappuoli, R;
Indirizzi:
Childrens Hosp, Oakland Res Inst, Oakland, CA 94609 USA Childrens Hosp Oakland CA USA 94609 kland Res Inst, Oakland, CA 94609 USA Inst Ric Immunobiol, Siena, Italy Inst Ric Immunobiol Siena ItalyInst Ric Immunobiol, Siena, Italy Univ Siena, Dept Biol Mol, I-53100 Siena, Italy Univ Siena Siena Italy I-53100 iena, Dept Biol Mol, I-53100 Siena, Italy
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 11, volume: 167, anno: 2001,
pagine: 6487 - 6496
SICI:
0022-1767(200112)167:11<6487:ANMAEP>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
OUTER-MEMBRANE PROTEIN; C CAPSULAR POLYSACCHARIDE; SEROGROUP-B; MONOCLONAL-ANTIBODIES; MENINGOCOCCAL VACCINE; BACILLUS-SUBTILIS; PEPTIDE LIBRARY; IMMUNE-RESPONSE; UNITED-STATES; EPITOPES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Granoff, DM Childrens Hosp, Oakland Res Inst, 5700 Martin Luther King Jr Way, Oakland,CA 94609 USA Childrens Hosp 5700 Martin Luther King Jr Way Oakland CA USA 94609
Citazione:
D.M. Granoff et al., "A novel mimetic antigen eliciting protective antibody to Neisseria meningitidis", J IMMUNOL, 167(11), 2001, pp. 6487-6496

Abstract

Molecular mimetic Ags are of considerable interest as vaccine candidates. Yet there are few examples of mimetic Ags that elicit protective Ab againsta pathogen, and the functional activity of anti-mimetic Abs has not been studied in detail. As part of the Neisseria meningitidis serogroup B genome sequencing project, a large number of novel proteins were identified. Herein, we provide evidence that genome-derived Ag 33 (GNA33), a lipoprotein with homology to Escherichia coli murein transglycosylase, elicits protective Ab to meningococci as a result of mimicking an epitope on loop 4 of porin A(PorA) in strains with serosubtype P1.2. Epitope mapping of a bactericidalanti-GNA33 mAb using overlapping peptides shows that the mAb recognizes peptides from GNA33 and PorA that share a QTP sequence that is necessary but not sufficient for binding. By How cytometry, mouse antisera prepared against rGNA33 and the anti-GNA33 mAb bind as well as an anti-PorA P1.2 mAb to the surface of eight of nine N. meningitidis serogroup B strains tested withthe P1.2 serosubtype. Anti-GNA33 Abs also are bactericidal for most P1.2 strains and, for susceptible strains, the activity of an anti-GNA33 mAb is similar to that of an anticapsular mAb but less active than an anti-P1.2 mAb. Anti-GNA Abs also confer passive protection against bacteremia in infant rats challenged with P1.2 strains. Thus, GNA33 represents one of the most effective immunogenic mimetics yet described. These results, demonstrate that molecular mimetics have potential as meningococcal vaccine candidates.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 04:27:34