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Titolo:
A novel NF-kappa B/Rel site in intron 1 cooperates with proximal promoter elements to mediate TNF-alpha-induced transcription of the human polymeric Ig receptor
Autore:
Schjerven, H; Brandtzaeg, P; Johansen, FE;
Indirizzi:
Univ Oslo, Rikshosp, Lab Immunohistochem & Immunopathol, Inst Pathol, N-0027 Oslo, Norway Univ Oslo Oslo Norway N-0027 unopathol, Inst Pathol, N-0027 Oslo, Norway
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 11, volume: 167, anno: 2001,
pagine: 6412 - 6420
SICI:
0022-1767(200112)167:11<6412:ANNBSI>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
NECROSIS-FACTOR-ALPHA; IMMUNOGLOBULIN RECEPTOR; SECRETORY COMPONENT; EPITHELIAL-CELLS; INTERFERON-GAMMA; MESSENGER-RNA; IMMUNOHISTOCHEMICAL EVALUATION; CARCINOEMBRYONIC ANTIGEN; PROTEIN-SYNTHESIS; PIGR GENE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Schjerven, H Univ Oslo, Rikshosp, Lab Immunohistochem & Immunopathol, InstPathol, N-0027 Oslo, Norway Univ Oslo Oslo Norway N-0027 st Pathol, N-0027 Oslo, Norway
Citazione:
H. Schjerven et al., "A novel NF-kappa B/Rel site in intron 1 cooperates with proximal promoter elements to mediate TNF-alpha-induced transcription of the human polymeric Ig receptor", J IMMUNOL, 167(11), 2001, pp. 6412-6420

Abstract

Secretory Abs constitute the first line of specific immune defense at mucosal surfaces. Such Abs are generated by the active transport of polymeric Ig (pIg) across secretory epithelia mediated by the pIgR, also known as transmembrane secretory component (SC). The proinflammatory cytokine TNF-alpha is a key mediator of host responses to infections, and it can stimulate protein synthesis-dependent transcriptional up-regulation of pIgR/SC in the HT-29 intestinal adenocarcinoma cell line. By reporter gene assay we identified a novel TNF-alpha -responsive region located within a 748-bp fragment inintron 1 of the human pIgRISC gene which depended on an NF-kappaB/Rel sitefor full responsiveness. EMSAs demonstrated preferential binding of the NF-kappaB/Rel family member p65 (RelA) to this DNA element after TNF-alpha stimulation, with weaker and more delayed binding of p50. Furthermore, the TNF-alpha -responsive region in intron I required cooperation with DNA elements located in the proximal promoter region of the gene. Mutational analysisdemonstrated that an IFN-stimuIated response element near the transcriptional start site in exon I was involved in the TNF-alpha responsiveness. Thus, DNA elements located >4 kb apart were found to cooperate in TNF-alpha -induced pIgR/SC up-regulation. The intronic TNF-alpha -responsive enhancer overlapped with a recently identified IL-4-responsive enhancer. Several intronic DNA elements found to be functionally important in the human gene are highly conserved between the human and mouse pIgRISC genes, suggesting the presence of a conserved cytokine-responsive enhancer region.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 20:03:55