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Titolo:
The IL-15R alpha chain signals through association with Syk in human B cells
Autore:
Bulanova, E; Budagian, V; Pohl, T; Krause, H; Durkop, H; Paus, R; Bulfone-Paus, S;
Indirizzi:
Res Ctr Borstel, Dept Immunol & Cell Biol, D-23845 Borstel, Germany Res Ctr Borstel Borstel Germany D-23845 l Biol, D-23845 Borstel, Germany Free Univ Berlin, Klinikum Benjamin Franklin, Dept Urol, D-12200 Berlin, Germany Free Univ Berlin Berlin Germany D-12200 pt Urol, D-12200 Berlin, Germany Free Univ Berlin, Klinikum Benjamin Franklin, Dept Pathol, D-12200 Berlin,Germany Free Univ Berlin Berlin Germany D-12200 t Pathol, D-12200 Berlin,Germany Univ Hamburg, Hosp Eppendorf, Dept Dermatol, D-20246 Hamburg, Germany UnivHamburg Hamburg Germany D-20246 Dermatol, D-20246 Hamburg, Germany
Titolo Testata:
JOURNAL OF IMMUNOLOGY
fascicolo: 11, volume: 167, anno: 2001,
pagine: 6292 - 6302
SICI:
0022-1767(200112)167:11<6292:TIACST>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-TYROSINE KINASE; ACTIVATED KILLER-CELLS; COMMON GAMMA-CHAIN; IL-2 RECEPTOR; GROWTH-FACTOR; BETA-CHAIN; SRC-FAMILY; INTERLEUKIN-2 RECEPTOR; ANTIGEN RECEPTOR; IN-VIVO;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
72
Recensione:
Indirizzi per estratti:
Indirizzo: Bulfone-Paus, S Res Ctr Borstel, Dept Immunol & Cell Biol, Pk Allee 22, D-23845 Borstel, Germany Res Ctr Borstel Pk Allee 22 Borstel Germany D-23845rmany
Citazione:
E. Bulanova et al., "The IL-15R alpha chain signals through association with Syk in human B cells", J IMMUNOL, 167(11), 2001, pp. 6292-6302

Abstract

The alpha -chain of the IL-15R (IL-15R alpha) serves as the specific, high-affinity receptor for IL-15. It is expressed by lymphoid and nonlymphoid cells, including B cell lymphoma lines. In this study, we have further explored IL-15R alpha -mediated signaling in activated primary B cells and in Raji cells, a human B-lymphoblastoid cell line which expresses the IL-15R alpha and IL-2R gamma chains, but lacks the IL-2R beta chain. Stimulation of Raji cells with IL-15 induces their proliferation and rescues them from C2-ceramide-induced apoptosis. By immunoprecipitation and Western blotting, we show that treatment of Raji cells and activated primary B cells with IL-15 induces coprecipitation of Syk kinase with the IL-15R alpha chain. Upon association, the activated Syk kinase phosphorylates the IL-15R alpha chain aswell as phospholipase C gamma, which coprecipitates with Syk. Furthermore,transfection of Raji cells with stem-loop Syk antisense oligonucleotides prevents IL-15R alpha and phospholipase C gamma phosphorylation as well as the inhibition of apoptosis by IL-15. Mutation of a defined region of the intracellular signaling portion of IL-15R alpha (Tyr(227)) abrogates both theIL-15R alpha /Syk association and IL-15R alpha phosphorylation. Taken together, this suggests that Syk kinase physically and functionally associates with the IL-15R alpha chain in B cells and that Syk plays a key role in mediating IL-15-induced signal transduction, thus accounting for the distinct functional consequences of IL-15 vs IL-2 binding to B cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/04/20 alle ore 15:16:13