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Titolo:
Development of oligonucleotide lateral-flow immunoassay for multi-parameter detection
Autore:
Oku, Y; Kamiya, K; Kamiya, H; Shibahara, Y; Ii, T; Uesaka, Y;
Indirizzi:
Nissui Pharmaceut Co Ltd, Div Res, Yuki, Ibaraki 3070036, Japan Nissui Pharmaceut Co Ltd Yuki Ibaraki Japan 3070036 baraki 3070036, Japan
Titolo Testata:
JOURNAL OF IMMUNOLOGICAL METHODS
fascicolo: 1-2, volume: 258, anno: 2001,
pagine: 73 - 84
SICI:
0022-1759(200112)258:1-2<73:DOOLIF>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTIGEN;
Keywords:
journal category; clinical immunology; oligonucleotide; immunochromatography; immunoassay; nitrocellulose; colloidal gold;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
7
Recensione:
Indirizzi per estratti:
Indirizzo: Oku, Y Nissui Pharmaceut Co Ltd, Div Res, 1075-2 Hokunanmoro, Yuki, Ibaraki 3070036, Japan Nissui Pharmaceut Co Ltd 1075-2 Hokunanmoro Yuki Ibaraki Japan 3070036
Citazione:
Y. Oku et al., "Development of oligonucleotide lateral-flow immunoassay for multi-parameter detection", J IMMUNOL M, 258(1-2), 2001, pp. 73-84

Abstract

We have developed a highly sensitive and rapid oligonucleotide lateral-flow immunoassay (OLFIA), using a colloidal gold as an indicator, for the simultaneous detection of antigens and/or antibodies in specimen. This system can detect more than two types of antigens and/or antibodies in a single assay device at the same time. The device is basically composed of colloidal gold-labeled antibodies and oligonucleotide-labeled antibodies fixed in a conjugate pad, and the complementary oligonucleotide-labeled proteins are immobilized on a nitrocellulose membrane. If the target antigen is present in a specimen, the colloidal gold-labeled antibody and oligonucleotide-labeledantibody will make a complex with the antigen. Subsequently, the formed complex migrates to the place where complementary oligonucleotide is immobilized and is bound to the solid phase via the DNA-DNA interaction. As a result, more than two types of reactions can be detected on a single assay device by the combination of colloidal gold-labeled antibodies, different oligonucleotide-labeled antibodies and complementary oligonucleotide-labeled proteins immobilized at different places on a nitrocellulose membrane. (C) 2001Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 06:19:22