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Titolo:
Immortalized Epstein-Barr virus-positive B-cell lines obtained by prolonged culture of peripheral blood mononuclear cells from human immunodeficiencyvirus type 1-positive patients
Autore:
Ruibal-Ares, B; Belmonte, L; Bare, P; Scolnik, M; Palacios, MF; Bayo-Hanza, C; Galmarini, CM; Mendez, G; de Bracco, MME;
Indirizzi:
Acad Nacl Med Buenos Aires, IIHEMA, RA-1425 Buenos Aires, DF, Argentina Acad Nacl Med Buenos Aires Buenos Aires DF Argentina RA-1425 F, Argentina
Titolo Testata:
JOURNAL OF HUMAN VIROLOGY
fascicolo: 4, volume: 4, anno: 2001,
pagine: 200 - 213
SICI:
1090-9508(200107/08)4:4<200:IEVBLO>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
NON-HODGKINS-LYMPHOMAS; MULTINUCLEATED GIANT-CELLS; HUMAN-HERPESVIRUS TYPE-8; AIDS-RELATED LYMPHOMAS; LYMPHOPROLIFERATIVE DISORDERS; KAPOSIS-SARCOMA; DNA-SEQUENCES; ATYPICAL LYMPHOPROLIFERATIONS; LATENT MEMBRANE-PROTEIN-1; DELETION VARIANTS;
Keywords:
B-cell lines; HIV-1 infection; EBV+ cell lines; HIV-1/EBV interaction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Ruibal-Ares, B Acad Nacl Med Buenos Aires, IIHEMA, P de Meio 3081, RA-1425Buenos Aires, DF, Argentina Acad Nacl Med Buenos Aires P de Meio 3081 Buenos Aires DF Argentina RA-1425
Citazione:
B. Ruibal-Ares et al., "Immortalized Epstein-Barr virus-positive B-cell lines obtained by prolonged culture of peripheral blood mononuclear cells from human immunodeficiencyvirus type 1-positive patients", J HUMAN VIR, 4(4), 2001, pp. 200-213

Abstract

Objectives: To study the factors that determine malignant B cell growth inhuman immunodeficiency virus type 1 (HIV-1)-infected patients. Study Design: B-cell lines (lymphocyte cell lines [LCL]) were developed after nonstimulated culture of peripheral blood mononuclear cells (PBMC) fromHIV-1-positive (HIV-1(+)) patients. Human immunodeficiency virus type I replication in culture, Epstein-Barr virus (EBV) latent oncogene expression, and cell-to-cell interaction were studied after nonstimulated culture of HIV-1(+) PBMC, analyzing their contribution to LCL appearance. Methods: Nonstimulated PBMC cultures of HIV-1+ PBMC and controls (N-PBMC) were established. Lymphocyte cell lines were characterized. Epstein-Barr virus latent membrane protein 1 (LMP-1) and Epstein-Barr nuclear antigen 2 were detected by polymerase chain reaction (PCR). Clonality of LCL was determined by light chain restriction (flow cytometry) and immunoglobulin H chainrearrangement (semi-nested PCR). Peripheral blood mononuclear cell phenotypes were studied at different intervals of culture, Results: Lymphocyte cell lines were obtained in 73% of HIV-1(+) PBMC cultures, compared with 6% inN-PBMC. Ali LCL were EBV-positive (EBV+). B-cell lineage was established, and up to 12 different B-cell clones were expanded from the same individual. Occurrence of LCL was more frequent in cultures with HIV-1 replication, high LMP-1 expression in viable B cells, and high CD4:CD8 ratio. Human immunodeficiency virus type 1 replication persisted in 53% of the LCL. Conclusions: In vitro HIV-1 replication and persistence of viable EBV+ lymphoblasts favor spontaneous in vitro outgrowth of LCL in HIV-1(+) patients.

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Documento generato il 08/04/20 alle ore 11:09:36