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Titolo:
High-performance liquid chromatography of HIV protease inhibitors in humanbiological matrices
Autore:
Aarnoutse, RE; Wissen, CPWGMVV; Underberg, WJM; Kleinnijenhuis, J; Hekster, YA; Burger, DM;
Indirizzi:
Univ Nijmegen, Med Ctr, Dept Clin Pharm, NL-6500 HB Nijmegen, Netherlands Univ Nijmegen Nijmegen Netherlands NL-6500 HB 0 HB Nijmegen, Netherlands Univ Utrecht, Fac Pharm, Dept Pharmaceut Anal, NL-3584 CA Utrecht, Netherlands Univ Utrecht Utrecht Netherlands NL-3584 CA 3584 CA Utrecht, Netherlands
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1-2, volume: 764, anno: 2001,
pagine: 363 - 384
SICI:
1387-2273(20011125)764:1-2<363:HLCOHP>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
SOLID-PHASE EXTRACTION; IMMUNODEFICIENCY-VIRUS INFECTION; MASS-SPECTROMETRIC DETECTION; HUMAN PLASMA; CEREBROSPINAL-FLUID; ANTIRETROVIRAL AGENTS; ULTRAVIOLET DETECTION; INDINAVIR; SAQUINAVIR; RITONAVIR;
Keywords:
reviews; protease inhibitors;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
84
Recensione:
Indirizzi per estratti:
Indirizzo: Aarnoutse, RE Univ Nijmegen, Med Ctr, Dept Clin Pharm, POB 9101, NL-6500 HB Nijmegen, Netherlands Univ Nijmegen POB 9101 Nijmegen Netherlands NL-6500 HB ands
Citazione:
R.E. Aarnoutse et al., "High-performance liquid chromatography of HIV protease inhibitors in humanbiological matrices", J CHROMAT B, 764(1-2), 2001, pp. 363-384

Abstract

Methods for HPLC analysis of protease inhibitors (Pls) in human biologicalmatrices were reviewed. Assays have been developed for analysis of single PIs or for simultaneous measurement of multiple PIs in plasma-serum, saliva, cerebrospinal fluid and semen. Liquid-liquid extraction was most often applied for sample pretreatment but solid-phase extraction and protein precipitation were used as well. Reversed-phase or ion-pair chromatography have been used to separate PIs. Detection of Pls should be sensitive enough for quantitation of plasma concentrations below trough levels of single Pls, or below proposed therapeutic thresholds for Pls. The large majority of assaysemploys UV detection. As the potential for interferences is large, the selectivity of every method should be evaluated properly. The available high-performance liquid chromatography (HPLC) methods have been applied in clinical pharmacokinetic studies and for therapeutic drag monitoring of Pls. Participation in an interlaboratory quality control program is recommended for every laboratory engaged in the bioanalysis of PIs. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/20 alle ore 14:00:50