Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Separation methods for acyclovir and related antiviral compounds
Autore:
Loregian, A; Gatti, R; Palu, G; De Palo, EF;
Indirizzi:
Univ Padua, Dept Med Diagnost Sci, I-35121 Padua, Italy Univ Padua PaduaItaly I-35121 pt Med Diagnost Sci, I-35121 Padua, Italy Univ Padua, Dept Histol Microbiol & Med Biotechnol, I-35121 Padua, Italy Univ Padua Padua Italy I-35121 ol & Med Biotechnol, I-35121 Padua, Italy Azienda Osped Padova, Dept Med & Surg Sci, I-35121 Padua, Italy Azienda Osped Padova Padua Italy I-35121 Surg Sci, I-35121 Padua, Italy
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1-2, volume: 764, anno: 2001,
pagine: 289 - 311
SICI:
1387-2273(20011125)764:1-2<289:SMFAAR>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; HERPES-SIMPLEX VIRUS; PREVENT CYTOMEGALOVIRUS DISEASE; CAPILLARY ZONE ELECTROPHORESIS; HIGH-DOSE ACYCLOVIR; L-VALYL ESTER; HUMAN-PLASMA; TRANSPLANT RECIPIENTS; THERAPEUTIC EFFICACY; CONTROLLED TRIAL;
Keywords:
reviews; acyclovir; valaciclovir; ganciclovir; penciclovir;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
128
Recensione:
Indirizzi per estratti:
Indirizzo: De Palo, EF Univ Padua, Dept Med Diagnost Sci, I-35121 Padua, Italy Univ Padua Padua Italy I-35121 ost Sci, I-35121 Padua, Italy
Citazione:
A. Loregian et al., "Separation methods for acyclovir and related antiviral compounds", J CHROMAT B, 764(1-2), 2001, pp. 289-311

Abstract

Acyclovir (ACV) is an antiviral drug, which selectively inhibits replication of members of the herpes group of DNA viruses with low cell toxicity. Valaciclovir (VACV), a prodrug of ACV is usually preferred in the oral treatment of viral infections, mainly herpes simplex virus (HSV). Also other analogues such as ganciclovir and penciclovir are discussed here. The former acts against cytomegalovirus (CMV) in general and the latter against CW retinitis. The action mechanism of these antiviral drugs is presented briefly here, mainly via phosphorylation and inhibition of the viral DNA polymerase. The therapeutic use and the pharmacokinetics are also outlined. The measurement of the concentration of acyclovir and related compounds in biological samples poses a particularly significant challenge because these drugs tendto be structurally similar to endogenous substances. The analysis requiresthe use of highly selective analytical techniques and chromatography methods are a first choice to determine drug content in pharmaceuticals and to measure them in body fluids. Chromatography can be considered the procedure of choice for the bio-analysis of this class of antiviral compounds, as this methodology is characterised by good specificity and accuracy and it is particularly useful when metabolites need to be monitored. Among chromatographic techniques, the reversed-phase (RP) HPLC is widely used for the analysis. C-18 Silica columns from 7.5 to 30 cm in length are used, the separation is carried out mainly at room temperature and less than 10 min is sufficient for the analysis at 1.0-1.5 ml/min of flow-rate. The separation methodsrequire an isocratic system, and various authors have proposed a variety of mobile phases. The detection requires absorbance or fluorescence measurements carried out at 250-254 nm and at lambda (ex) = 260-285 nm, lambda (em)= 375-380 nm, respectively. The detection limit is about 0.3-10 ng/ml but the most important aspect is related to the sample treatment, mainly when body fluids are under examination. The plasma samples obtained from human blood are pre-treated with an acid or acetonitrile deproteinization and the supernatant after centrifugation is successively extracted before RP-HPLC injection. Capillary Electrophoresis methods are also discussed. This new analytical approach might be the expected evolution, in fact the analyses are improved with regard to time and performance, in particular coated capillary as well as addition of stabilisers have been employed. The time of analysis is shortened arriving at less than half a minute. Furthermore by using an electrochemical detection, and having a calibration linearity in the range of 0.2-20.0 ng/ml, the detection limit is 0.15 mug/ml. The measurements of acyclovir arid penciclovir have been presented but in the future other related drugs will probably be available using CE methods. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 20:07:40