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Titolo:
High performance liquid chromatographic analysis of St. John's Wort with photodiode array detection
Autore:
Li, WK; Fitzloff, JF;
Indirizzi:
Univ Illinois, Coll Pharm, Dept Med Chem & Pharmacognosy MC 781, FFH Core Analyt Lab,Program Collaborat Res Pharma, Chicago, IL 60612 USA Univ Illinois Chicago IL USA 60612 orat Res Pharma, Chicago, IL 60612 USA
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 765, anno: 2001,
pagine: 99 - 105
SICI:
1387-2273(200112)765:1<99:HPLCAO>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
FORCED SWIMMING TEST; HYPERICUM-PERFORATUM; ANTIDEPRESSANT ACTIVITY; CLINICAL-TRIALS; HYPERFORIN; EXTRACTS; PSEUDOHYPERICIN; DEPRESSION; COMPONENTS; MODELS;
Keywords:
St. John's Wort; Hypericum perforatum; flavonoids; phloroglucinols; naphthodianthrones;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Fitzloff, JF Univ Illinois, Coll Pharm, Dept Med Chem & Pharmacognosy MC 781, FFH Core Analyt Lab,Program Collaborat Res Pharma, PCRPS,M-C 877,833 S Wood St, Chicago, IL 60612 USA Univ Illinois PCRPS,M-C 877,833 S Wood St Chicago IL USA 60612
Citazione:
W.K. Li e J.F. Fitzloff, "High performance liquid chromatographic analysis of St. John's Wort with photodiode array detection", J CHROMAT B, 765(1), 2001, pp. 99-105

Abstract

An RP-HPLC method with photodiode array detection was established for the determination of major constituents (rutin, hyperoside, isoquercitrin, quercitrin, quercetin, pseudohypericin, hyperforin and hypericin) in St. John'sWort dietary supplements. The samples were extracted with methanol by means of sonication in low temperature. The extraction was rapid, with two steps of sonication (30 min each) recovering more than 99% of the major constituents in St. John's Wort samples. The major components were separated by RP-18 chromatography column using a 60-min water-acetonitrile-methanol-trifluoroacetic acid gradient. The quantification was performed by using externalstandards. Sample preparation and stability of methanolic extract of St. John's Wort were extensively explored. It is worth noting that the major constituents in the methanolic extract of St John's Wort, especially hypericinand pseudohypericin, might be retained by some filter cartridges during the filtration. The current method may serve as a valuable tool for the QA/QCof St. John's Wort dietary supplements. (C) 2001 Elsevier Science B.V. Allrights reserved.

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Documento generato il 20/01/20 alle ore 08:16:59