Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Direct chiral assay of tramadol and detection of the phase II metabolite O-demethyl tramadol glucuronide in human urine using capillary electrophoresis with laser-induced native fluorescence detection
Autore:
Soetebeer, UB; Schierenberg, MO; Schulz, H; Andresen, P; Blaschke, G;
Indirizzi:
Univ Munster, Inst Pharmaceut Chem, D-48149 Munster, Germany Univ MunsterMunster Germany D-48149 ceut Chem, D-48149 Munster, Germany Univ Bielefeld, Fac Phys, D-33615 Bielefeld, Germany Univ Bielefeld Bielefeld Germany D-33615 hys, D-33615 Bielefeld, Germany
Titolo Testata:
JOURNAL OF CHROMATOGRAPHY B
fascicolo: 1, volume: 765, anno: 2001,
pagine: 3 - 13
SICI:
1387-2273(200112)765:1<3:DCAOTA>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
SIMULTANEOUS STEREOSELECTIVE ANALYSIS; LIQUID-CHROMATOGRAPHIC DETERMINATION; HUMAN PLASMA; GAS-CHROMATOGRAPHY; MASS-SPECTROMETRY; HUMAN LIVER; ENANTIOMERS; PHARMACOLOGY; EXTRACTION;
Keywords:
tramadol; O-demethyl tramadol glucuronide;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Blaschke, G Univ Munster, Inst Pharmaceut Chem, Hittorfstr 58-62, D-48149 Munster, Germany Univ Munster Hittorfstr 58-62 Munster Germany D-48149 Germany
Citazione:
U.B. Soetebeer et al., "Direct chiral assay of tramadol and detection of the phase II metabolite O-demethyl tramadol glucuronide in human urine using capillary electrophoresis with laser-induced native fluorescence detection", J CHROMAT B, 765(1), 2001, pp. 3-13

Abstract

A chiral separation using carboxymethyl-beta -cyclodextrin and methyl-beta-cyclodextrin for the direct assay of tramadol in human urine by capillaryelectrophoresis (CE) with laser-induced native fluorescence detection was developed. Furthermore, the phase II metabolite O-demethyl tramadol glucuronide was determined from the urine samples and the ratio of the diasteromers was determined. The chiral method was validated. Correlation coefficientswere higher than 0.999. Within day variation showed accuracy in the range 96.1-105.8% with a RSD less than 6.00%. Day to day variation present an accuracy ranging from 100.2 to 103.5% with a RSD less than 5.4%. After oral administration of 150 mg tramadol hydrochloride to a healthy volunteer, the urinary excretion was monitored during 24 h. About 11.4% of the dose was excreted as 1S,2S-tramadol, 16.4% as 1R,2R-tramadol and 23.7% as O-demethyl tramadol glucuronide. The amount of 1S,2S O-demethyl tramadol glucuronide wasmore than three fold higher as 1R,2R-O-demethyl tramadol glucuronide. The enantiomeric ratio of tramadol and the diastereomeric ratio of O-demethyl tramadol glucuronide was deviated from 1.0 showing that a stereoselective metabolism of tramadol occurs. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 18:18:21