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Titolo:
The solution structure of [d(CGC)r(a(m)a(m)a(m))d(TTTGCG)](2)
Autore:
Tsao, YP; Wang, LY; Hsu, ST; Jain, ML; Chou, SH; Huang, WC; Cheng, JW;
Indirizzi:
Natl Tsing Hua Univ, Inst Life Sci, Div Struct Biol & Biomed Sci, Hsinchu 300, Taiwan Natl Tsing Hua Univ Hsinchu Taiwan 300 & Biomed Sci, Hsinchu 300, Taiwan Natl Taiwan Normal Univ, Dept Chem, Taipei, Taiwan Natl Taiwan Normal Univ Taipei Taiwan l Univ, Dept Chem, Taipei, Taiwan Natl Chunghsing Univ, Inst Biochem, Taichung 400, Taiwan Natl Chunghsing Univ Taichung Taiwan 400 t Biochem, Taichung 400, Taiwan
Titolo Testata:
JOURNAL OF BIOMOLECULAR NMR
fascicolo: 3, volume: 21, anno: 2001,
pagine: 209 - 220
SICI:
0925-2738(200111)21:3<209:TSSO[>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
RNA HYBRID DUPLEX; CHIRAL PHOSPHOROTHIOATE MOIETY; DNA DUPLEXES; MOLECULAR-STRUCTURE; 2'-HYDROXYL GROUP; CRYSTAL-STRUCTURE; ESCHERICHIA-COLI; B-DNA; NMR; JUNCTIONS;
Keywords:
2 '-OMe RNA; DNA center dot RNA; NMR; solution structure;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Cheng, JW Natl Tsing Hua Univ, Inst Life Sci, Div Struct Biol & Biomed Sci, Hsinchu 300, Taiwan Natl Tsing Hua Univ Hsinchu Taiwan 300 ci, Hsinchu 300, Taiwan
Citazione:
Y.P. Tsao et al., "The solution structure of [d(CGC)r(a(m)a(m)a(m))d(TTTGCG)](2)", J BIOM NMR, 21(3), 2001, pp. 209-220

Abstract

The solution structure and hydration of a DNA . RNA hybrid chimeric duplex[d(CGC)r(a(m)a(m)a(m))d(TTTGCG)](2) in which the RNA adenines were substituted by 2'-O-methylated riboadenines was determined using two-dimensional NMR, simulated annealing, and restrained molecular dynamics. Only DNA residue 7T in the 2'-OMe-RNA . DNA junction adopted an O4'-endo sugar conformation, while the other DNA residues including 3C in the DNA .2'-OMe-RNA junction, adopted C1'-exo or C2'-endo conformations. The observed NOE intensity of2'-O-methyl group to H1' proton of 4a(m) at the DNA . 2'-OMe-RNA junction is much weaker than those of 5a(m) and 6a(m). The 2'-O-methyl group of 4a(m) was found to orient towards the minor groove in the trans domain while the 2'-O- methyl groups of 5a(m) and 6a(m) were found to be in the gauche (+)domain. In contrast to the long-lived water molecules found close to the RNA adenine H2 and H1' protons and the methyl group of 7T in the RNA-DNA junction of [d(CGC)r(aaa)d(TTTGCG)](2), there were no long-lived water molecules found in [d(CGC)r(a(m)a(m)a(m))d(TTTGCG)](2). This is probably due to the hydrophobic enviroment created by the 2'-O-methylated riboadenines in theminor groove or due to the wider minor groove width in the middle of the structure. In addition, the 2'-O-methylation of riboadenines in pure chimeric duplex increses its melting temperature from 48.5 degreesC to 51.9 degreesC. The characteristic structural features and hydration patterns of this chimeric duplex provide a molecular basis for further therapeutic applications of DNA . RNA hybrid and chimeric duplexes with 2'-modified RNA residues.

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Documento generato il 01/12/20 alle ore 07:52:35