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Titolo:
Internalin B activates nuclear factor-kappa B via Ras, phosphoinositide 3-kinase, and Akt
Autore:
Mansell, A; Khelef, N; Cossart, P; ONeill, LAJ;
Indirizzi:
Univ Dublin Trinity Coll, Dept Biochem, Dublin 2, Ireland Univ Dublin Trinity Coll Dublin Ireland 2 ept Biochem, Dublin 2, Ireland Univ Dublin Trinity Coll, Inst Biotechnol, Dublin 2, Ireland Univ Dublin Trinity Coll Dublin Ireland 2 Biotechnol, Dublin 2, Ireland Inst Pasteur, Unite Interact Bacteries Cellules, Paris 15, France Inst Pasteur Paris France 15 teract Bacteries Cellules, Paris 15, France
Titolo Testata:
JOURNAL OF BIOLOGICAL CHEMISTRY
fascicolo: 47, volume: 276, anno: 2001,
pagine: 43597 - 43603
SICI:
0021-9258(20011123)276:47<43597:IBANFB>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIN-BASED MOVEMENT; LISTERIA-MONOCYTOGENES; PHOSPHATIDYLINOSITOL 3-KINASE; MAMMALIAN-CELLS; GROWTH-FACTOR; TYROSINE KINASE; INVASION PROTEIN; T-LYMPHOCYTES; INIB PROTEIN; RECEPTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: O'Neill, LAJ Univ Dublin Trinity Coll, Dept Biochem, Dublin 2, Ireland Univ Dublin Trinity Coll Dublin Ireland 2 Dublin 2, Ireland
Citazione:
A. Mansell et al., "Internalin B activates nuclear factor-kappa B via Ras, phosphoinositide 3-kinase, and Akt", J BIOL CHEM, 276(47), 2001, pp. 43597-43603

Abstract

Internalin B (InIB), a 630-amino acid protein loosely attached to the surface of Listeria monocytogenes, participates in the entry of the bacterium into mammalian cells. This process requires the activation of phosphoinositide (Pl) 3-kinase by InIB. Previously, we demonstrated that InIB activates the transcription factor Nuclear Factor-kappaB in murine J774 macrophage-like cells, an event that also requires PI 3-kinase. Here we have further investigated this phenomenon. InIB activated the small G-protein Ras in J774 cells. Inhibition of Ras with the farnesyltransferase inhibitor manumycin A inhibited NF-kappaB activation and the recruitment of the p85 subunit of PI 3-kinase, implying that Ras is required for PI 3-kinase activation. InIB also activated the PI 3-kinase downstream effector, Akt, as assessed by increased phosphorylation of Akt on serine 473. Transfection of Hep2 cells with dominant negative Ras N17 or dominant negative Akt inhibited the induction of a reporter gene linked to the interleukin-8 promoter by InIB. Furthermore, the Ras inhibitor manumycin A, the PI 3-kinase inhibitor LY294002, and an Akt inhibitor all blocked the induction of interleukin-8 by InIB. Our study is the first report of a bacterial product activating a pathway involving Ras, PI 3-kinase, and Akt, which leads to NF-kappaB activation. This process could be involved in host defense or the inhibition of apoptosis duringinfection.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 08:01:42