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Titolo:
Expression and interaction of different catenins in colorectal carcinoma cells
Autore:
Kucerova, D; Sloncova, E; Tuhackova, Z; Vojtechova, M; Sovova, V;
Indirizzi:
Acad Sci Czech Republ, Inst Genet Mol, Prague 16637 6, Czech Republic AcadSci Czech Republ Prague Czech Republic 16637 6 37 6, Czech Republic
Titolo Testata:
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
fascicolo: 6, volume: 8, anno: 2001,
pagine: 695 - 698
SICI:
1107-3756(200112)8:6<695:EAIODC>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
APC TUMOR-SUPPRESSOR; BETA-CATENIN; E-CADHERIN; TRANSCRIPTION FACTOR; ALPHA-CATENIN; ADHESION; COMPLEX; PHOSPHORYLATION; ACTIVATION; MUTATIONS;
Keywords:
beta-catenin; p120 catenin; colorectal carcinoma cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Sovova, V Acad Sci Czech Republ, Inst Genet Mol, Flemingovo Nam 2, Prague 16637 6, Czech Republic Acad Sci Czech Republ Flemingovo Nam 2 Prague Czech Republic 16637 6
Citazione:
D. Kucerova et al., "Expression and interaction of different catenins in colorectal carcinoma cells", INT J MOL M, 8(6), 2001, pp. 695-698

Abstract

Aberrant signalling activities of beta -catenin, originally identified as a component of cell-adhesion complexes, are now considered to be an important factor in colorectal carcinogenesis. However, recently it was shown thatalso gamma- as well as p120 catenins have a dual role either in cell adhesion or in affecting some gene activation. Therefore, the levels and interactions of these three catenins in human colorectal carcinoma cell lines wereanalysed. A great heterogeneity in the expression of all catenins tested was found in colorectal carcinoma cell lines HT29 and LS174T. Detailed analysis of beta -catenin interactions was done. GST-APC fragment-fused proteinswere used to absorb beta -catenin and its complexes from cell lysates. Similarly, the E-cadherin binding capacity of the residual pool of beta -catenin was analysed using the GST-ECT construct. It was found that the level ofbeta -catenin does not necessarily depend either on the APC or beta -catenin gene mutations and that co-precipitation of beta-, gamma-, and p120 catenins is not limited to cells that express E-cadherin.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 11:00:26