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Titolo:
Stimulatory effect of zinc on insulin-like growth factor-I and transforming growth factor-beta 1 production with bone growth of newborn rats
Autore:
Ma, ZJ; Misawa, H; Yamaguchi, M;
Indirizzi:
Univ Shizouka, Grad Sch Nutr Sci, Lab Endocrinol & Mol Metab, Shizuoka 4228526, Japan Univ Shizouka Shizuoka Japan 4228526 Mol Metab, Shizuoka 4228526, Japan
Titolo Testata:
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
fascicolo: 6, volume: 8, anno: 2001,
pagine: 623 - 628
SICI:
1107-3756(200112)8:6<623:SEOZOI>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
L-HISTIDINATO ZINC; PROTEIN-SYNTHESIS; TISSUE-CULTURE; OVARIECTOMIZED RATS; FACTOR-BETA; RESORPTION; RNA; COMPONENTS; METABOLISM; CELLS;
Keywords:
bone growth; zinc; insulin-like growth factor-I; transforming growth factor-beta; newborn rat femur;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Yamaguchi, M Univ Shizouka, Grad Sch Nutr Sci, Lab Endocrinol & Mol Metab,52-1 Yada, Shizuoka 4228526, Japan Univ Shizouka 52-1 Yada Shizuoka Japan4228526 228526, Japan
Citazione:
Z.J. Ma et al., "Stimulatory effect of zinc on insulin-like growth factor-I and transforming growth factor-beta 1 production with bone growth of newborn rats", INT J MOL M, 8(6), 2001, pp. 623-628

Abstract

The effect of zinc, an essential trace element, on insulin-like growth factor-I (IGF-I) and transforming growth factor-beta1 production was investigated to determine the role of this metal in bone growth of newborn rats. Femoraldiaphyseal and metaphyseal tissues were obtained between 1 and 28 days after birth of newborn rats, and cultured for 24 h in a serum-free Dulbecco's modified Eagle's medium containing either vehicle or zinc sulfate (10(-6)-10(-4) M). Protein concentration in the medium was significantly increased by culture with bone tissues of newborn rats with increasing age (14 and 21 days). Medium IGF-I and TGF-beta1 concentration was gradually reduced with increasing age after birth. The presence of zinc (10(-5) and 10(-4) M) caused a significant increase in protein, IGF-I, and TCTF-beta1 concentrations in the medium cultured with the diaphyseal or metaphyseal tissues obtained at 7 and 14 days after birth. The expression of IGF-I and TGF-beta1 mRNAwas demonstrated by reverse transcription-polymerase chain reaction (RT-PCR) analysis in the diaphyseal and metaphyseal tissues cultured for 24 h using rat IGF-I or TGF-beta1-specific primers. These expressions were significantly raised in the presence of zinc (10-4 M) in culture medium. The present study demonstrates that zinc has a stimulatory effect on IGF-I and TGF-beta1 production in the femoral tissues with bone growth of newborn rats.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 01:05:20