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Titolo:
Activated platelets and endothelial cell interaction with neutrophils under flow conditions
Autore:
Mine, S; Fujisaki, T; Suematsu, M; Tanaka, Y;
Indirizzi:
Univ Occupat & Environm Hlth, Sch Med, Dept Internal Med 1, Yahatanishi Ku, Kitakyushu, Fukuoka 8078555, Japan Univ Occupat & Environm Hlth Kitakyushu Fukuoka Japan 8078555 8555, Japan
Titolo Testata:
INTERNAL MEDICINE
fascicolo: 11, volume: 40, anno: 2001,
pagine: 1085 - 1092
SICI:
0918-2918(200111)40:11<1085:APAECI>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLYCOPROTEIN-IIB-IIIA; P-SELECTIN; VONWILLEBRAND-FACTOR; LEUKOCYTE ADHESION; IN-VIVO; RECEPTOR; INTEGRIN; BINDING; THROMBIN; INTERLEUKIN-8;
Keywords:
neutrophil; platelet; endothelium; GPIIb/IIIa; integrin; P-selectin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Mine, S Univ Occupat & Environm Hlth, Sch Med, Dept Internal Med 1, Yahatanishi Ku, 1-1 Iseigaoka, Kitakyushu, Fukuoka 8078555, Japan Univ Occupat & Environm Hlth 1-1 Iseigaoka Kitakyushu Fukuoka Japan 8078555
Citazione:
S. Mine et al., "Activated platelets and endothelial cell interaction with neutrophils under flow conditions", INTERN MED, 40(11), 2001, pp. 1085-1092

Abstract

Objective The mechanism of neutrophil adhesion to the endothelium during the earliest stages of acute inflammation, especially before the induction of adhesion molecules on endothelial cells, remains unknown. We studied the possible involvement of platelets in this process. Methods Neutrophils were added to human umbilical vein-derived endothelialcells (HUVEC) with or without adherent platelets in the presence or absence of adhesion-blocking monoclonal antibodies (mAbs). Adhesion of neutrophils to HUVEC at dynamic flow conditions was assessed using a flow chamber. Results 1) Thrombin-activated platelets adhered to resting-HUVEC at dynamic flow conditions through platelet glycoprotein Ilb/IIIa and RGD proteins. 2) Neutrophils tethered to P-selectin induced on thrombin-activated platelets, which were immobilized on HUVEC. 3) Activated neutrophils adhered, via LFA-1, to ICAM-1 on HUVEC. 4) Activated platelets induced interleukin (IL)-8 secretion by HUVEC. Conclusions Immobilized platelets on the vessel wall with induced P-selectin on the surface biochemically and functionally promote the adhesion of neutrophils to endothelial cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/08/20 alle ore 17:57:51