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Titolo:
Fish monocytes as a model for mycobacterial host-pathogen interactions
Autore:
El-Etr, SH; Yan, L; Cirillo, JD;
Indirizzi:
Univ Nebraska, Dept Vet & Biomed Sci, Lincoln, NE 68583 USA Univ NebraskaLincoln NE USA 68583 et & Biomed Sci, Lincoln, NE 68583 USA
Titolo Testata:
INFECTION AND IMMUNITY
fascicolo: 12, volume: 69, anno: 2001,
pagine: 7310 - 7317
SICI:
0019-9567(200112)69:12<7310:FMAAMF>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
MACROPHAGE-LIKE CELLS; GAMMA-INTERFERON; LEGIONELLA-PNEUMOPHILA; ALLELIC EXCHANGE; LEUKEMIA-CELLS; ANIMAL-MODEL; TUBERCULOSIS; MARINUM; ACTIVATION; AVIUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
77
Recensione:
Indirizzi per estratti:
Indirizzo: Cirillo, JD Univ Nebraska, Dept Vet & Biomed Sci, Lincoln, NE 68583 USA Univ Nebraska Lincoln NE USA 68583 Sci, Lincoln, NE 68583 USA
Citazione:
S.H. El-Etr et al., "Fish monocytes as a model for mycobacterial host-pathogen interactions", INFEC IMMUN, 69(12), 2001, pp. 7310-7317

Abstract

Mycobacterium marinum, a relatively rapid-growing fish and human pathogen,has become an important model for the investigation of mycobacterial pathogenesis. Al. marinum is closely related to the Mycobacterium tuberculosis complex and causes a disease in fish and amphibians with pathology similar to tuberculosis. We have developed an in vitro model for the study of M. marinum virulence mechanisms using the carp monocytic cell line CLC (carp leukocyte culture). We found that fish monocytes can differentiate between pathogenic and nonpathogenic mycobacterial species. Interestingly, M. marinum enters fish monocytes at a 40- to 60-fold-higher rate than Mycobacterium smegmatis. In addition, M. marinum survives and replicates in fish monocytes while M. smegmatis is killed. We also found that M. marinum inhibits lysosomal fusion in fish monocytes, indicating that these cells may be used to dissect the mechanisms of intracellular trafficking in mycobacteria. We conclude from these observations that monocytic cells from fish, a natural host for Al. marinum, provide an extremely valuable model for the identification and characterization of mycobacterial virulence determinants in the laboratory.

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Documento generato il 02/07/20 alle ore 17:57:42