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Titolo:
Plant regeneration through somatic embryogenesis and RAPD analysis of regenerated plants in Tylophora indica (Burm. f. Merrill.)
Autore:
Jayanthi, M; Mandal, PK;
Indirizzi:
MS Swaminathan Res Fdn, Taramani 600113, Chennai, India MS Swaminathan ResFdn Taramani Chennai India 600113 0113, Chennai, India
Titolo Testata:
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
fascicolo: 5, volume: 37, anno: 2001,
pagine: 576 - 580
SICI:
1054-5476(200109/10)37:5<576:PRTSEA>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
CALLUS-CULTURES; STABILITY; MARKERS;
Keywords:
medicinal plant; callus induction; somatic embryos; plant regeneration; DNA isolation; genetic homogeneity;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Jayanthi, M Trop Bot Garden & Res Inst, Plant Biotechnol Div, Thiruvananthapuram, Kerala, India Trop Bot Garden & Res Inst Thiruvananthapuram KeralaIndia a
Citazione:
M. Jayanthi e P.K. Mandal, "Plant regeneration through somatic embryogenesis and RAPD analysis of regenerated plants in Tylophora indica (Burm. f. Merrill.)", IN VITRO-PL, 37(5), 2001, pp. 576-580

Abstract

A procedure for the regeneration of complete plantlets of Tylophora indica, from cultured leaf Callus via somatic embryogenesis is described. Callus induction from leaf explants was on Murashige and Skoog (MS) medium with different concentrations of 2,4-dichlorophenoxy-acetic acid (2,4-D; 0.0-3 mg l(-1); 0.0-13.56 muM) and kinetin (Kn; 0-01 mg l(-1); 0.05 muM). The best response for callus induction was obtained on MS medium containing 2 mg l(-1) (9.04 muM) 2,4-D and 0.01 mg l(-1) (0.05. muM), Kn. After two subcultureson the same medium the embryogenic Callus was transferred to MS medium with different concentrations of the cytokinin, 6-benzyladenine (0.5-3 mg l(-1); 2.22-13.32 muM) and 2-isopentenyladenine (2ip; 0.53 mg l(-1); 2.46-14.76muM) along with 0.01 mg l(-1) (0.05 muM) indole-3-butyric acid (IBA) for somatic embryo development and maturation. MS medium with 2 mg l(-1) (9.84 muM) 2ip produced the maximum number of mature somatic embryos. The mature embryos were bipolar and on transfer to MS basal medium produced complete plantlets. After hardening the regenerants were planted in the Gudalur forests of Western Chats. Total DNA was extracted from 14 regenerants and the mother plant. Random amplified polymorphic DNA (RAPD) analysis was carried outusing 20, arbitrary oligonucleotides. The amplification products were monomorphic among all the plants revealing the genetic homogeneity and true-to-type nature of the regenerants.

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Documento generato il 28/11/20 alle ore 14:26:40