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Titolo:
A unique basal pattern of p53 expression in ulcerative colitis is associated with mutation in the p53 gene
Autore:
Noffsinger, AE; Belli, JM; Miller, MA; Fenoglio-Preiser, CM;
Indirizzi:
Univ Cincinnati, Med Ctr, Dept Pathol & Lab Med, Cincinnati, OH 45267 USA Univ Cincinnati Cincinnati OH USA 45267 Lab Med, Cincinnati, OH 45267 USA
Titolo Testata:
HISTOPATHOLOGY
fascicolo: 5, volume: 39, anno: 2001,
pagine: 482 - 492
SICI:
0309-0167(200111)39:5<482:AUBPOP>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR SUPPRESSOR GENE; CANCER CELL-LINES; RAS MUTATIONS; P53-INDEPENDENT PATHWAY; COLORECTAL-CANCER; POINT MUTATIONS; LEUKEMIA-CELLS; CYCLE ARREST; DIFFERENTIATION; INDUCTION;
Keywords:
inflammatory bowel disease; ulcerative colitis; dysplasia; immunohistochemistry; p2l; p53;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Noffsinger, AE Univ Cincinnati, Med Ctr, Dept Pathol & Lab Med, POB 670529, Cincinnati, OH 45267 USA Univ Cincinnati POB 670529 Cincinnati OH USA 45267 5267 USA
Citazione:
A.E. Noffsinger et al., "A unique basal pattern of p53 expression in ulcerative colitis is associated with mutation in the p53 gene", HISTOPATHOL, 39(5), 2001, pp. 482-492

Abstract

Aims: The p53 protein is implicated in the control of cell proliferation. differentiation, and death. As part of a study characterizing p53 alterations in colonic mucosa of patients with ulcerative colitis. we identified a unique pattern of basal p53 immunoreactivity. Methods and results: Tissue samples (n=180) from 42 ulcerative colitis patients were evaluated for p53 alterations by immunohistochemistry, loss of heterozygosity analysis. polymerase chain reaction-single-strand conformation polymorphism and direct sequencing. In addition. the expression of the p53-associated proteins p21(waf1/cip1) and MDM2 was evaluated immunohistochemically. Three basic patterns of p53 immunoreactivity were observed: (i) isolated immunoreactive cells in the crypt bases: (ii) strongly positive cellsconfined to the basal half of the glands: and (iii) diffusely staining cells. The basal staining pattern was observed in both non-neoplastic tissues and in some areas of dysplasia, and was associated with normal expression of p21(waf1/cip1) in all cases, and with p53 mutation in seven of 11 cases. Conclusions: The basal pattern of p53 expression is associated with mutation in the p53 gene, and appears to be an early change in a subgroup of ulcerative colitis patients. The significance of this pattern of immunoreactivity and the mechanism by which it develops are discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/01/20 alle ore 09:30:15