Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Mammalian homologue of E-coli ras-like GTPase (ERA) is a possible apoptosis regulator with RNA binding activity
Autore:
Akiyama, T; Gohda, J; Shibata, S; Nomura, Y; Azuma, S; Ohmori, Y; Sugano, S; Arai, H; Yamamoto, T; Inoue, J;
Indirizzi:
Keio Univ, Fac Sci & Technol, Dept Appl Chem, Kohoku Ku, Yokohama, Kanagawa 2238522, Japan Keio Univ Yokohama Kanagawa Japan 2238522 kohama, Kanagawa 2238522, Japan Univ Tokyo, Grad Sch Pharmaceut Sci, Dept Hlth Chem, Bunkyo Ku, Tokyo 1130033, Japan Univ Tokyo Tokyo Japan 1130033 lth Chem, Bunkyo Ku, Tokyo 1130033, Japan Univ Tokyo, Inst Med Sci, Dept Virol, Minato Ku, Tokyo 1088639, Japan UnivTokyo Tokyo Japan 1088639 pt Virol, Minato Ku, Tokyo 1088639, Japan Univ Tokyo, Inst Med Sci, Dept Oncol, Minato Ku, Tokyo 1088639, Japan UnivTokyo Tokyo Japan 1088639 pt Oncol, Minato Ku, Tokyo 1088639, Japan
Titolo Testata:
GENES TO CELLS
fascicolo: 11, volume: 6, anno: 2001,
pagine: 987 - 1001
SICI:
1356-9597(200111)6:11<987:MHOERG>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVATED PROTEIN-KINASE; FRAGILE-X-SYNDROME; 16S RIBOSOMAL-RNA; ESCHERICHIA-COLI; SIGNAL-TRANSDUCTION; KH-DOMAIN; MUTATIONAL ANALYSIS; INITIATION CODONS; SPLICING FACTOR; GENE ENCODES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
67
Recensione:
Indirizzi per estratti:
Indirizzo: Inoue, J Keio Univ, Fac Sci & Technol, Dept Appl Chem, Kohoku Ku, Yokohama, Kanagawa 2238522, Japan Keio Univ Yokohama Kanagawa Japan 2238522 anagawa 2238522, Japan
Citazione:
T. Akiyama et al., "Mammalian homologue of E-coli ras-like GTPase (ERA) is a possible apoptosis regulator with RNA binding activity", GENES CELLS, 6(11), 2001, pp. 987-1001

Abstract

Background: ERA (Escherichia coli Ras-like protein) is an E. coli GTP binding protein that is essential for proliferation. A DNA database search suggests that homologous sequences with ERA exist in various organisms including human, mouse, Drosophila, Caenorhabditis elegans and Antirrhinum majus. However, the physiological function of eukaryotic ERA-like proteins is not known. Results: We have cloned cDNAs encoding the entire coding region of a humanhomologue (H-ERA) and a mouse homologue (M-ERA) of ERA. The mammalian homologue of ERA consists of a typical GTPase/GTP-binding domain and a putativeK homology (KH) domain, which is known as an RNA binding domain. We performed transfection experiments with wild-type H-ERA or various H-ERA mutants. H-ERA possessing the amino acid substitution mutation into the GTPase domain induced apoptosis of HeLa cells, which was blocked by Bcl-2 expression. Deletion of the C-terminus, which contains a part of the KH domain, alleviated apoptosis by the H-ERA mutant, suggesting the importance of this domainin the function of H-ERA. We have also shown the RNA binding activity of H-ERA by pull-down experiments using RNA homopolymer immobilized on beads orrecombinant H-ERA proteins. Conclusion: Our data suggest that H-ERA plays an important role in the regulation of apoptotic signalling with its GTPase/GTP binding domain.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 11:21:04