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Titolo:
Food allergy - towards predictive testing for novel foods
Autore:
Oehlschlager, S; Reece, P; Brown, A; Hughson, E; Hird, H; Chisholm, J; Atkinson, H; Meredith, C; Pumphrey, R; Wilson, P; Sunderland, J;
Indirizzi:
Cent Sci Lab, York YO41 1LZ, N Yorkshire, England Cent Sci Lab York N Yorkshire England YO41 1LZ 1LZ, N Yorkshire, England British Ind Biol Res Assoc, TNO, Carshalton SM5 4DS, Surrey, England British Ind Biol Res Assoc Carshalton Surrey England SM5 4DS rey, England St Marys Hosp, Reg Immunol Dept, Manchester M13 0JH, Lancs, England St Marys Hosp Manchester Lancs England M13 0JH er M13 0JH, Lancs, England
Titolo Testata:
FOOD ADDITIVES AND CONTAMINANTS
fascicolo: 12, volume: 18, anno: 2001,
pagine: 1099 - 1107
SICI:
0265-203X(200112)18:12<1099:FA-TPT>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
FC-EPSILON-RI; BIRCH POLLEN ALLERGENS; MAST-CELLS; CROSS-REACTIVITY; IGE; PROTEIN; ALPHA; SENSITIZATION; DERMATITIS; CHILDREN;
Keywords:
food allergy; IgE; database; modelling; 2D electrophoresis; digestion model; cell based assay; in vivo;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Oehlschlager, S Cent Sci Lab, York YO41 1LZ, N Yorkshire, England Cent SciLab York N Yorkshire England YO41 1LZ e, England
Citazione:
S. Oehlschlager et al., "Food allergy - towards predictive testing for novel foods", FOOD ADDIT, 18(12), 2001, pp. 1099-1107

Abstract

The risks associated with IgE-mediated food allergy highlight the need formethods to screen for potential food allergens. Clinical and immunologicaltests are available for the diagnosis of food allergy to known food allergens, but this does not extend to the evaluation, or prediction of allergenicity in novel foods. This category includes foods produced using novel processes, genetically modified (GM) foods, and foods that might be used as alternatives to traditional foods. Through the collation and analysis of the protein sequences of known allergens and their epitopes, it is possible to identify related groups which correlate with observed clinical cross-reactivities. 3-D modelling extends the use of sequence data and can be used to display eptiopes on the surface of a molecule. Experimental models support sequence analysis and 3-D modelling. Observed crossreactivities can be examined by Western blots prepared from native 2-D gels of a whole food preparation (e.g. hazelnut, peanut), and common proteins identified. IgEs to novel proteins can be raised in Brown Norway rat (a high IgE responder strain), and the proteins tested in simulated digest to determine epitope stability. Using the CSL serum bank, epitope binding can be examined through the ability of an allergen to cross-link the high affinity IgE receptor and thereby release mediators using in vitro cell-based models. This range of methods, in combination with data mining, provides a variety of screening options fortesting the potential of a novel food to be allergenic, which does not involve prior exposure to the consumer.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 18:08:58