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Titolo:
Following the course of human leptospirosis: evidence of a critical threshold for the vital prognosis using a quantitative PCR assay
Autore:
Truccolo, J; Serais, O; Merien, F; Perolat, P;
Indirizzi:
Inst Pastuer Nouvelle Caledonie, Leptospira Lab, Noumea 98845, New Caledonia Inst Pastuer Nouvelle Caledonie Noumea New Caledonia 98845 New Caledonia
Titolo Testata:
FEMS MICROBIOLOGY LETTERS
fascicolo: 2, volume: 204, anno: 2001,
pagine: 317 - 321
SICI:
0378-1097(20011113)204:2<317:FTCOHL>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; INFECTION; DIAGNOSIS;
Keywords:
leptospirosis; Leptospira interrogans; quantitative polymerase chain reaction; pathogenesis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
11
Recensione:
Indirizzi per estratti:
Indirizzo: Perolat, P Inst Pastuer Nouvelle Caledonie, Leptospira Lab, POB 61, Noumea98845, NewCaledonia Inst Pastuer Nouvelle Caledonie POB 61 Noumea New Caledonia 98845
Citazione:
J. Truccolo et al., "Following the course of human leptospirosis: evidence of a critical threshold for the vital prognosis using a quantitative PCR assay", FEMS MICROB, 204(2), 2001, pp. 317-321

Abstract

In order to follow the course of acute human leptospirosis, an ELISA microtiter plate hybridization method was developed for the quantitative determination of Leptospira spp. in biological samples after PCR. The biotin-labelled amplified product (331 bp from the rrs ae, ne) was hybridized with a complementary capture probe covalently linked onto animated polystyrene wells, and detected using a colorimetric reaction. The mean detection limit was 50 copies per 10 mul. In a prospective study of human leptospirosis cases, we obtained evidence that a density of 10(4) leptospires per ml of blood isa critical threshold for the vital prognosis of the patients. The practicability of the method makes it suitable for use in tropical areas for multicentric studies. Such Studies could lead to a better knowledge of the natural history of the human disease. The method is also suitable for experimental evaluation of improved antibiotic treatments for leptospirosis. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 09:47:46