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Titolo:
Increased detection of interleukin-5 in sputum by addition of protease inhibitors
Autore:
Kelly, MM; Leigh, R; Carruthers, S; Horsewood, P; Gleich, GJ; Hargreave, FE; Cox, G;
Indirizzi:
McMaster Univ, St Josephs Hosp, Dept Med, Asthma Res Grp, Hamilton, ON, Canada McMaster Univ Hamilton ON Canada d, Asthma Res Grp, Hamilton, ON, Canada McMaster Univ, St Josephs Hosp, Dept Pathol, Hamilton, ON, Canada McMasterUniv Hamilton ON Canada Hosp, Dept Pathol, Hamilton, ON, Canada Mayo Clin, Sch Med, Dept Immunol, Rochester, MN USA Mayo Clin Rochester MN USA lin, Sch Med, Dept Immunol, Rochester, MN USA
Titolo Testata:
EUROPEAN RESPIRATORY JOURNAL
fascicolo: 4, volume: 18, anno: 2001,
pagine: 685 - 691
SICI:
0903-1936(200110)18:4<685:IDOIIS>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
AIRWAY INFLAMMATION; ASTHMATIC-PATIENTS; CYTOKINES; IL-5; CELL; INDEXES; PREDNISONE; ALLERGEN; VALIDITY; KINETICS;
Keywords:
asthma; inflammation; interleukin-5; proteases; protease inhibitors; sputum chemistry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Cox, G St Josephs Hosp, Firestone Inst Resp Hlth, 50 Charlton Ave E, Hamilton, ONL8N 4A6, Canada St Josephs Hosp 50 Charlton Ave E Hamilton ON CanadaL8N 4A6 Canada
Citazione:
M.M. Kelly et al., "Increased detection of interleukin-5 in sputum by addition of protease inhibitors", EUR RESP J, 18(4), 2001, pp. 685-691

Abstract

The measurement of interleukin (IL)-5 in sputum is problematic, with interfering factors affecting immunoassay. The authors investigated whether sputum proteases could he acting as interfering factors by studying the effect of protease inhibitors (PI) on sputum IL-5 measurement. Induced sputa from 20 subjects with asthma were divided into aliquots, processed with and without protease inhibitors (in low and high concentrations) and the levels of IL-5 (spiked and endogenous) measured by enzyme immunoassay were compared. The concentration of sputum IL-5 was significantly increased by PI, with median (interquartile range) levels processed with no, low and high PI concentrations being 0 (0), 41.8 (75.6) and 66.1 (124.4) pg.mL(-1), respectively. There was also a significant increase in percentage recovery of spiked IL-5. Although high concentrations of PI reduced cell viability, there was noeffect on total or differential cell counts and low concentrations of PI had no effect on cell counts or viability. Levels of endogenous interleukin-5 in sputum of asthmatic subjects can be significantly increased by the addition of protease inhibitors, and sampleswhich would be regarded as negative for interleukin-5 without protease inhibitors may instead have considerable amounts of interleukin-5 detected.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/10/20 alle ore 00:41:36