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Titolo:
Immunohistochemical detection of 8-hydroxy-2 '-deoxyguanosine in gallbladder epithelium of patients with pancreaticobiliary maljunction
Autore:
Otani, K; Shimizu, S; Chijiiwa, K; Yamaguchi, K; Noshiro, H; Tanaka, M;
Indirizzi:
Kyushu Univ, Fac Med, Dept Endoscop Diagnost & Therapeut, Higashi Ku, Fukuoka 8128582, Japan Kyushu Univ Fukuoka Japan 8128582 ut, Higashi Ku, Fukuoka 8128582, Japan
Titolo Testata:
EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY
fascicolo: 11, volume: 13, anno: 2001,
pagine: 1363 - 1369
SICI:
0954-691X(200111)13:11<1363:IDO8'I>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
OXIDATIVE DNA-DAMAGE; K-RAS; ANOMALOUS JUNCTION; DUCTAL JUNCTION; POINT MUTATIONS; CARCINOMA; 8-HYDROXYDEOXYGUANOSINE; CARCINOGENESIS; STRESS; BASE;
Keywords:
carcinogenesis; DNA damage; gallbladder; 8-hydroxy-2 '-deoxyguanosine; 4-hydroxy-2-nonenal; immunohistochemistry; oxidative stress; p53; pancreaticobiliary maljunction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Shimizu, S Kyushu Univ, Fac Med, Dept Endoscop Diagnost & Therapeut, Higashi Ku, 3-1-1 Maidashi, Fukuoka 8128582, Japan Kyushu Univ 3-1-1 Maidashi Fukuoka Japan 8128582 128582, Japan
Citazione:
K. Otani et al., "Immunohistochemical detection of 8-hydroxy-2 '-deoxyguanosine in gallbladder epithelium of patients with pancreaticobiliary maljunction", EUR J GASTR, 13(11), 2001, pp. 1363-1369

Abstract

Objectives Pancreaticobiliary maljunction, an anomalous union of the pancreatic duct with the common bile duct, is a risk factor for biliary carcinoma. We hypothesized that, in patients with pancreaticobiliary maljunction, persistent regurgitation of pancreatic juice into the biliary tract induces oxidative DNA damage. We assessed the expression of an oxidative DNA base-modified product, 8-hydroxy-2'-deoxyguanosine, in gallbladder epithelium. Design Eleven noncancerous gallbladders from patients with pancreaticobiliary maljunction, 12 gallbladder carcinomas from patients without pancreaticobiliary maljunction and 14 noncancerous gallbladders from patients withoutpancreaticobiliary maljunction (control) were studied. Methods Immunohistochemistry was performed for 4-hydroxy-2-nonenal-modified protein (as a marker for lipid peroxidation), 8-hydroxy-2'-deoxyguanosineand p53 gene product. Results Stronger cytoplasmic staining of 4-hydroxy-2-nonenal-modified protein was observed in the gallbladder epithelium from patients with pancreaticobiliary maljunction than in epithelium from gallbladder cancer patients or from control subjects with normal gallbladders. Clear, strong nuclear staining of 8-hydroxy-2'-deoxyguanosine was observed in the gallbladder epithelial cells from patients with pancreaticobiliary maljunction. Densitometricquantitation revealed significantly higher expression of 8-hydroxy-2'-deoxyguanosine in gallbladder epithelial cells from patients with pancreaticobiliary maljunction (index 27.3 +/- 3.1) than in cells from patients with gallbladder carcinoma (11.4 +/- 1.5; P < 0.05) or from control subjects with normal gallbladder (6.4 +/- 1.0; P < 0.05). Positivity of p53 was 27% in gallbladder epithelium associated with pancreaticobiliary maljunction, 75% ih gallbladder carcinoma epithelium and 0% in control epithelium. Conclusions These results suggest that reactive oxygen species are produced in the gallbladder of patients with pancreaticobiliary maljunction and that oxidative DNA injury is related to carcinogenesis in these patients. (C)2001 Lippincott Williams & Wilkins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 09:29:01