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Titolo:
Epithelial cell-derived components induce transforming growth factor-alphamRNA expression and epithelial cell proliferation in vitro
Autore:
Hoffmann, P; Dignass, AU; Zeeh, JM; Mazurkiewicz, J; Holtmann, G; Gerken, G;
Indirizzi:
Ruhr Univ Bochum, St Josef Hosp, Dept Med 1, D-44791 Bochum, Germany Ruhr Univ Bochum Bochum Germany D-44791 t Med 1, D-44791 Bochum, Germany
Titolo Testata:
EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY
fascicolo: 11, volume: 13, anno: 2001,
pagine: 1333 - 1340
SICI:
0954-691X(200111)13:11<1333:ECCITG>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXPERIMENTAL COLITIS; EXPERIMENTAL-MODEL; FACTOR-RECEPTOR; GASTRIC-MUCOSA; FACTOR-BETA; BLOOD-FLOW; RATS; MIGRATION; INTESTINE; LIPOPOLYSACCHARIDE;
Keywords:
epithelial cell function; gastrointestinal mucosal injury; transforming growth factor-alpha;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Hoffmann, P Ruhr Univ Bochum, St Josef Hosp, Dept Med 1, Gudrunstr 56, D-44791 Bochum,Germany Ruhr Univ Bochum Gudrunstr 56 Bochum Germany D-44791 ,Germany
Citazione:
P. Hoffmann et al., "Epithelial cell-derived components induce transforming growth factor-alphamRNA expression and epithelial cell proliferation in vitro", EUR J GASTR, 13(11), 2001, pp. 1333-1340

Abstract

Background Epidermal growth factor (EGF) and transforming growth factor (TGF)-alpha protect the gastrointestinal mucosa against injury. In response to mucosal injury TGF-alpha, but not EGF, is locally increasingly expressed in the mucosa of the rat colon and stomach 4-8 h after injury. The aim of our present study was to characterize the possible signal for the induction of TGF-alpha expression. Methods Monolayers of the non-transformed intestinal epithelial cell (IEC)-6 and IEC-18 lines were harvested, homogenized and shock-frozen at -80 degreesC for 1 h. Cell cultures of intact IEC-6 or IEC-18 cells were exposed to these cell homogenates and modulation of epithelial cell migration and proliferation was evaluated using standardized procedures as described previously. TGF-alpha mRNA expression in the exposed epithelial cell monolayers was assessed using reverse transcriptase-polymerase chain reaction (RT-PCR) and normalized to the expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Results The proliferation of IEC-6 and IEC-18 epithelial cell monolayers was significantly inhibited if epithelial cell homogenates of 2000 cells/ml or more, and significantly induced if homogenates of less than 2000 cells/ml, were applied to the medium of the monolayers in vitro. In proliferating epithelial cells, a significant two-fold increase in TGF-alpha mRNA expression was obtained at 48 h and 72 h after application of the cell homogenates. The homogenate-induced epithelial cell proliferation was completely abolished after preincubation of the epithelial cell homogenates with neutralizing monoclonal anti-TGF-a antibodies. No effect on epithelial cell migrationwas noticed after application of epithelial-cell homogenates to the cell cultures. Conclusions Epithelial cell-derived components induce TGF-alpha mRNA expression and TGF-alpha -dependent cell proliferation of intact epithelial cells. We hypothesize that epithelial cell interaction bears one of the possible signals for the increased TGF-alpha mRNA expression after mucosal injury. (C) 2001 Lippincott Williams & Wilkins.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 07:46:44