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Titolo:
Adrenalectomy regulates apoptotic-associated genes in rat hippocampus
Autore:
Greiner, M; Cardenas, S; Parra, C; Bravo, J; Avalos, AM; Paredes, A; Lara, HE; Fiedler, JL;
Indirizzi:
Univ Chile, Fac Chem & Pharmaceut Sci, Dept Biochem & Mol Biol, Lab Neurobiochem, Santiago 1, Chile Univ Chile Santiago Chile 1 ol Biol, Lab Neurobiochem, Santiago 1, Chile
Titolo Testata:
ENDOCRINE
fascicolo: 3, volume: 15, anno: 2001,
pagine: 323 - 333
SICI:
1355-008X(200108)15:3<323:ARAGIR>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; TUMOR-SUPPRESSOR P53; CORTICOSTEROID RECEPTOR BALANCE; GRANULE CELL DEGENERATION; DENTATE GYRUS; BCL-X; IN-VIVO; GLOBAL-ISCHEMIA; BAX PROTEIN; ADULT-RAT;
Keywords:
adrenalectomy; corticosterone; apoptosis; Bax; Bcl-X-L; Bcl-2;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Fiedler, JL Univ Chile, Fac Chem & Pharmaceut Sci, Dept Biochem & Mol Biol, Lab Neurobiochem, POB 233, Santiago 1, Chile Univ Chile POB 233 SantiagoChile 1 OB 233, Santiago 1, Chile
Citazione:
M. Greiner et al., "Adrenalectomy regulates apoptotic-associated genes in rat hippocampus", ENDOCRINE, 15(3), 2001, pp. 323-333

Abstract

Morphological studies of granular neurons of the hippocampus have shown that adrenalectomy (ADX) induces the cell death of granular neurons, an effect prevented by corticosterone replacement. We addressed the hypothesis thatcorticosterone regulates the expression of the apoptotic bcl-2 gene family. Five days after adrenalectomy, we observed morphological changes related to hippocampal granule cell apoptosis that was accompanied by terminal dUTPnick and labeling (TUNEL) labeling in nuclei located in the hilus region. Corticosterone replacement prevented the cell death induced by ADX. Using RT-PCR we found a reduction in mRNA levels of the antiapoptotic gene bcl-2 in whole hippocampus, an effect which was prevented by corticosterone administration to ADX rats. However, Bcl-2 protein levels were not altered by this treatment. We did not observe modifications in the level of bcl-X-L mRNA however, we did find a 40% reduction in Bcl-X-L protein levels, an effect not reversed by corticosterone. In contrast, we found a reduction in the mRNA of the antiapoptotic gene bax and Bax levels after ADX; both effects wereprevented by corticosterone. The reduction in proapoptotic bax and in antiapoptotic bcl-2 mRNA levels in the whole hippocampus, suggests that local variations in these molecules could account for both neuronal viability of the CA1-CA3 and granular cell death detected by morphological means and observed after ADX.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 22:08:56