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Titolo:
CHARACTERIZATION OF IL-12 RECEPTOR BETA-1 CHAIN (IL-12R-BETA-1)-DEFICIENT MICE - IL-12R-BETA-1 IS AN ESSENTIAL COMPONENT OF THE FUNCTIONAL-MOUSE IL-12 RECEPTOR
Autore:
WU CY; FERRANTE J; GATELY MK; MAGRAM J;
Indirizzi:
HOFFMANN LA ROCHE INC,DEPT INFLAMMAT AUTOIMMUNE DIS,340 KINGSLAND ST NUTLEY NJ 07110 HOFFMANN LA ROCHE INC,DEPT INFLAMMAT AUTOIMMUNE DIS NUTLEY NJ 07110
Titolo Testata:
The Journal of immunology
fascicolo: 4, volume: 159, anno: 1997,
pagine: 1658 - 1665
SICI:
0022-1767(1997)159:4<1658:COIRBC>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVATED HUMAN LYMPHOBLASTS; STEM-CELL LINES; CD4+ T-CELLS; BIOLOGIC ACTIVITY; INTERLEUKIN-12; EXPRESSION; CLONING; CYTOKINE; BINDING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
C.Y. Wu et al., "CHARACTERIZATION OF IL-12 RECEPTOR BETA-1 CHAIN (IL-12R-BETA-1)-DEFICIENT MICE - IL-12R-BETA-1 IS AN ESSENTIAL COMPONENT OF THE FUNCTIONAL-MOUSE IL-12 RECEPTOR", The Journal of immunology, 159(4), 1997, pp. 1658-1665

Abstract

Two chains of the IL-12R, IL-12R beta 1 and IL-12R beta 2, have recently been cloned, To determine the role of IL-12R beta 1 in mediating the biologic functions of IL-12 in mice, we have generated IL-12R beta 1-deficient (IL-12R beta 1(-/-)) mice by targeted mutation in ES cells, Con A-activated splenocytes from IL-12R beta 1(+/+) mice displayed both high and low affinity IL-12-binding sites, whereas Con A-activatedsplenocytes from IL-12R beta 1(-/-) mice expressed only low affinity IL-12-binding sites, Consistent with the expression of low affinity IL-12-binding sites on IL-12R beta 1(-/-) lymphoblasts, these cells expressed normal amounts of IL-12R beta 2 mRNA. Unlike those from IL-12R beta 1(+/+) mice, Con A-activated splenocytes from IL-12R beta 1(-/-) mice failed to proliferate or produce IFN-gamma in response to IL-12, even at very high concentrations (67 nM). In contrast, lymphoblasts from both types of mice proliferated equally well to IL-2 or IL-7. Splenocytes from IL-12R beta 1(-/-) mice also failed to display enhanced NK lytic activity when cultured with IL-12 but responded normally to IL-2, Similar to IL-12 p40-deficient mice, IL-12R beta 1(-/-) mice were impaired in their ability to produce IFN-gamma in response to endotoxin administration in vivo, and IL-12R beta 1(-/-) splenocytes were deficient in IFN-gamma secretion when stimulated with either Con A or soluble anti-CD3 mAb in vitro, These results demonstrate that IL-12R beta 1 is required for mouse T and NK cells to respond to IL-12 and that expression of low affinity IL-12-binding sites, presumably reflecting expression of IL-12R beta 2, is by itself insufficient to mediate IL-12 responsiveness, even in the presence of very high concentrations of IL-12.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/09/20 alle ore 04:15:06