Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Molecular identification and characterization of turkey IFN-gamma gene
Autore:
Loa, CC; Hsieh, MK; Wu, CC; Lin, TL;
Indirizzi:
Purdue Univ, Dept Vet Pathobiol, W Lafayette, IN 47907 USA Purdue Univ W Lafayette IN USA 47907 Pathobiol, W Lafayette, IN 47907 USA
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
fascicolo: 4, volume: 130, anno: 2001,
pagine: 579 - 584
SICI:
1096-4959(200112)130:4<579:MIACOT>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHICKEN INTERFERON; MACROPHAGES;
Keywords:
bioassay; chicken; interferon-gamma; macrophages; turkey; nitric oxide induction; cross-species reactivity; neutralization; genomic relationship;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
9
Recensione:
Indirizzi per estratti:
Indirizzo: Lin, TL Purdue Univ, Dept Vet Pathobiol, W Lafayette, IN 47907 USA Purdue Univ W Lafayette IN USA 47907 l, W Lafayette, IN 47907 USA
Citazione:
C.C. Loa et al., "Molecular identification and characterization of turkey IFN-gamma gene", COMP BIOC B, 130(4), 2001, pp. 579-584

Abstract

The cDNAs of turkey and chicken interferon gamma (IFN-gamma) were cloned and the functional activity of turkey and chicken IFN-gamma was compared. The coding region of turkey IFN-gamma gene encodes a predicted mature proteinof 145 amino acids with a molecular weight at 16.8 kDa. Compared with typeI IFN, the IFN-gamma between turkey and chicken also had the same size andhigh degree of identity at the nucleotide (96.0%) and amino acid (96.4%) sequence. Turkey IFN-gamma was cross-reactive with chicken cells. Both turkey and chicken IFN-gamma could induce production of nitric oxide by turkey or chicken macrophages. Turkey IFN-gamma also had similar degree of sensitivity to heat and pH 2.0 as chicken IFN-gamma. The functional activity of both turkey and chicken IFN-gamma could be neutralized by a monoclonal antibody specific to chicken IFN-gamma to a similar extent. These results indicated that IFN-gamma protein was cross-reactive between turkey and chicken. (C)2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/07/20 alle ore 07:59:46