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Titolo:
Opening of mitochondrial K-ATP channels attenuates the ouabain-induced calcium overload in mitochondria
Autore:
Ishida, H; Hirota, Y; Genka, C; Nakazawa, H; Nakaya, H; Sato, T;
Indirizzi:
Chiba Univ, Grad Sch Med, Dept Pharmacol, Chuo Ku, Chiba 2608670, Japan Chiba Univ Chiba Japan 2608670 Pharmacol, Chuo Ku, Chiba 2608670, Japan Tokai Univ, Sch Med, Dept Physiol, Isehara, Kanagawa 25911, Japan Tokai Univ Isehara Kanagawa Japan 25911 l, Isehara, Kanagawa 25911, Japan
Titolo Testata:
CIRCULATION RESEARCH
fascicolo: 10, volume: 89, anno: 2001,
pagine: 856 - 858
SICI:
0009-7330(20011109)89:10<856:OOMKCA>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEPENDENT POTASSIUM CHANNELS; CARDIAC MYOCYTES; INHIBITION;
Keywords:
mitochondria; calcium; K-ATP channel; cardioprotection;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
12
Recensione:
Indirizzi per estratti:
Indirizzo: Sato, T Chiba Univ, Grad Sch Med, Dept Pharmacol, Chuo Ku, Inohana 1-8-1, Chiba 2608670, Japan Chiba Univ Inohana 1-8-1 Chiba Japan 2608670 Chiba 2608670, Japan
Citazione:
H. Ishida et al., "Opening of mitochondrial K-ATP channels attenuates the ouabain-induced calcium overload in mitochondria", CIRCUL RES, 89(10), 2001, pp. 856-858

Abstract

We tested whether opening of mitochondrial ATP-sensitive K+ (mitoK(ATP)) channels depolarizes mitochondrial membrane potential (Delta Psi (m)) and thereby prevents the mitochondrial Ca2+ overload. With the use of a Nipkow disk confocal system, the mitochondrial Ca2+ concentration ([Ca2+](m)) and Delta Psi (m) in rat ventricular myocytes were measured by loading cells withRhod-2 and JC-1, respectively. Exposure to ouabain (1 mmol/L) for 30 minutes produced mitochondrial Ca2+ overload, and the intensity of Rhod-2 fluorescence significantly increased to 173 +/- 16% of baseline (P <0.001). Treatment of myocytes with the mitoK(ATP) channel opener diazoxide (100 mu mol/L) blunted the ouabain-induced mitochondrial Ca2+ overload (131 +/- 10% of baseline; P <0.001 versus ouabain). Moreover, diazoxide significantly depolarized the Delta Psi (m). and reduced the intensity of JC-1 fluorescence during application of ouabain to 89 +/-2% of baseline (P <0.05). These effectsof diazoxide were blocked by the mitoK(ATP) channel blocker 5-hydroxydecanoate (500 mu mol/L). These results indicate that opening of mitoK(ATP) channels prevents a mitochondrial Ca2+ overload in association with Delta Psi (m). depolarization and thereby protects myocardium against ischernic damage.

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Documento generato il 29/03/20 alle ore 18:00:51