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Titolo:
Molecular cytogenetic characterization of the EBV-producing cell line B95-8 (Saguinus oedipus, Platyrrhini) by chromosome sorting and painting
Autore:
Muller, S; Neusser, M; OBrien, PCM; Wienberg, J;
Indirizzi:
Univ Munich, Inst Anthropol & Human Genet, D-80539 Munich, Germany Univ Munich Munich Germany D-80539 Human Genet, D-80539 Munich, Germany Univ Cambridge, Dept Pathol, Cambridge CB2 1QP, England Univ Cambridge Cambridge England CB2 1QP hol, Cambridge CB2 1QP, England
Titolo Testata:
CHROMOSOME RESEARCH
fascicolo: 8, volume: 9, anno: 2001,
pagine: 689 - 693
SICI:
0967-3849(2001)9:8<689:MCCOTE>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPSTEIN-BARR VIRUS; MARMOSET LEUKOCYTES; KARYOTYPE; PRIMATES;
Keywords:
Epstein-Bar virus; Saguinus oedipus;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
8
Recensione:
Indirizzi per estratti:
Indirizzo: Wienberg, J Univ Munich, Inst Anthropol & Human Genet, D-80539 Munich, Germany Univ Munich Munich Germany D-80539 , D-80539 Munich, Germany
Citazione:
S. Muller et al., "Molecular cytogenetic characterization of the EBV-producing cell line B95-8 (Saguinus oedipus, Platyrrhini) by chromosome sorting and painting", CHROMOS RES, 9(8), 2001, pp. 689-693

Abstract

The cell line B95-8 releases Epstein-Barr virus (EBV) with high titres of transforming activity and is widely used as a model in cancer research and virology. There are, however, controversial reports about the species of origin, cell line stability and karyotype. To address these questions, B95-8 chromosomes were analysed by chromosome sorting and painting by multicolourfluorescence in-situ hybridization. Reciprocal painting was performed between B95-8, 'wildtype' New World monkey and human chromosomes. Saguinus oedipus was revealed as the species of origin. A further five cell-line-specific marker chromosomes, resulting from translocations, deletions and an insertion were found. Although human chromosome 6 or 13 homologues were always involved in these rearrangements, co-hybridization of an EBV-specific DNA probe did not reveal site-specific hybridization to marker chromosomes or at translocation breakpoints. The multicolour probe set described here will beof special value for further evolutionary studies in New World monkeys.

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Documento generato il 02/06/20 alle ore 01:54:23