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Titolo:
Effect of 5 '-deletion of Arabidopsis profilin2 promoter on its vascular specific expression
Autore:
Liu, YH; Wang, ZX; Jia, SR;
Indirizzi:
Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R ChinaChinese Acad Agr Sci Beijing Peoples R China 100081 081, Peoples R China
Titolo Testata:
CHINESE SCIENCE BULLETIN
fascicolo: 19, volume: 46, anno: 2001,
pagine: 1627 -
SICI:
1001-6538(200110)46:19<1627:EO5'OA>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE;
Keywords:
profilin2; promoter; Kalanchoe; constitutive expression; vascular-specific expression;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
10
Recensione:
Indirizzi per estratti:
Indirizzo: Jia, SR Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, PeoplesR China Chinese Acad Agr Sci Beijing Peoples R China 100081 ples R China
Citazione:
Y.H. Liu et al., "Effect of 5 '-deletion of Arabidopsis profilin2 promoter on its vascular specific expression", CHIN SCI B, 46(19), 2001, pp. 1627

Abstract

Based on the published sequence of profilin2 promoter of Arabidopsis thaliana, a full-length promoter (1667 bp) was amplified by PCR. The 5'-end deletion fragments with length of 1380, 1153, 969 and 597 bp were then fused with gus (uidA) gene respectively. Constructed plant expression vectors were individually transferred into Kalanchoe laciniata and transgenic plants regenerated. GUS histochemical assay confirmed that the full-length promoter Pfn1.7 was vascular-specific. Deletion assays showed that profilin2 promotercould be divided into three parts. Deletion of fragment 1 (-1667 - -1380 bp) resulted in constitutive expression, suggesting that element(s) responsible for vascular-specific expression might exist in this region. Fragment 2located at -1153 - -597 lip strongly inhibited gus gene expression. Fragment 3 ( -597 - -1 bp) is considered as a basic domain of profilin2.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 22:19:20