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Titolo:
Partially folded conformations in the folding pathway of bovine carbonic anhydrase II: A fluorescence spectroscopic analysis
Autore:
Bushmarina, NA; Kuznetsova, IM; Biktashev, AG; Turoverov, KK; Uversky, VN;
Indirizzi:
Russian Acad Sci, Inst Cytol, St Petersburg 194064, Russia Russian Acad Sci St Petersburg Russia 194064 t Petersburg 194064, Russia Univ Calif Santa Cruz, Dept Chem & Biochem, Santa Cruz, CA 95064 USA Univ Calif Santa Cruz Santa Cruz CA USA 95064 m, Santa Cruz, CA 95064 USA Russian Acad Sci, Inst Biol Instrumentat, Pushchino 142292, Moscow Region,Russia Russian Acad Sci Pushchino Moscow Region Russia 142292 scow Region,Russia
Titolo Testata:
CHEMBIOCHEM
fascicolo: 11, volume: 2, anno: 2001,
pagine: 813 - 821
SICI:
1439-4227(20011105)2:11<813:PFCITF>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOLTEN GLOBULE STATE; GUANIDINE-HYDROCHLORIDE; CRYSTAL-STRUCTURE; D-GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE; CHLAMYDOMONAS-REINHARDTII; INTRAMOLECULAR MOBILITY; PROBING SUBSTRUCTURE; CIRCULAR-DICHROISM; CREATINE-KINASE; PIG-HEART;
Keywords:
carbonic anhydrase; fluorescence spectroscopy; intermediates; lyases; protein folding;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Turoverov, KK Russian Acad Sci, Inst Cytol, Tikhoretsky Ave 4, St Petersburg 194064, Russia Russian Acad Sci Tikhoretsky Ave 4 St Petersburg Russia 194064
Citazione:
N.A. Bushmarina et al., "Partially folded conformations in the folding pathway of bovine carbonic anhydrase II: A fluorescence spectroscopic analysis", CHEMBIOCHEM, 2(11), 2001, pp. 813-821

Abstract

GdmCl-, urea-, and pH-induced unfolding pathways of bovine carbonic anhydrase II have been analyzed by using changes induced by different denaturing agents in intensity anisotropy, life time, and parameter A value of intrinsic fluorescence as well as intensity and life time of ANS (ammonium salt of8-anilinonaphthalene-1-sulfonic acid) fluorescence. The formation of several stable unfolding intermediates, some of which were not observed previously has been established, This was further confirmed by representation of fluorescence data in terms of a "phase diagram", that is, I-lambda1 versus I-lambda2 dependence, where I-lambda1 and I-lambda2 are the fluorescence intensity values measured at wavelengths lambda (1) and lambda (2), respectively.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/08/20 alle ore 16:57:53