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Titolo:
Neuroendocrine differentiation of the LNCaP prostate cancer cell line maintains the expression and function of VIP and PACAP receptors
Autore:
Juarranz, MG; Bolanos, O; Gutierrez-Canas, I; Lerner, EA; Robberecht, P; Carmena, MJ; Prieto, JC; Rodriguez-Henche, N;
Indirizzi:
Univ Alcala de Henares, Dept Biochem & Mol Biol, E-28871 Alcala De Henares, Spain Univ Alcala de Henares Alcala De Henares Spain E-28871 De Henares, Spain Harvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Dermatol, Charlestown,MA 02129 USA Harvard Univ Charlestown MA USA 02129 Dermatol, Charlestown,MA 02129 USA Free Univ Brussels, Sch Med, Dept Biochem & Nutr, B-1070 Brussels, BelgiumFree Univ Brussels Brussels Belgium B-1070 utr, B-1070 Brussels, Belgium
Titolo Testata:
CELLULAR SIGNALLING
fascicolo: 12, volume: 13, anno: 2001,
pagine: 887 - 894
SICI:
0898-6568(200112)13:12<887:NDOTLP>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYCLASE-ACTIVATING POLYPEPTIDE; VASOACTIVE-INTESTINAL-PEPTIDE; RAT VENTRAL PROSTATE; BENIGN HYPERPLASTIC PROSTATE; ISOLATED EPITHELIAL-CELLS; ADENYLATE-CYCLASE; MOLECULAR-CLONING; SPLICE VARIANTS; SIGNAL-TRANSDUCTION; CARCINOMA-CELLS;
Keywords:
neuroendocrine cells; transdifferentiation; prostate cancer; cAMP; VIP receptors; PACAP receptors;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
49
Recensione:
Indirizzi per estratti:
Indirizzo: Prieto, JC Univ Alcala de Henares, Dept Biochem & Mol Biol, E-28871 AlcalaDe Henares, Spain Univ Alcala de Henares Alcala De Henares Spain E-28871 Spain
Citazione:
M.G. Juarranz et al., "Neuroendocrine differentiation of the LNCaP prostate cancer cell line maintains the expression and function of VIP and PACAP receptors", CELL SIGNAL, 13(12), 2001, pp. 887-894

Abstract

The molecular mechanisms involved in differentiation of prostate cancer cells to a neuroendocrine (NE) cell phenotype are not well understood. Here we used the androgen-dependent human prostate cancer cell line LNCaP to perform a systematic and broad analysis of the expression, pharmacology, and functionality of vasoactive intestinal peptide (VIP)/pituitary adenylate cyclase-activating peptide (PACAP) receptors. Reverse transcription polymerase chain reaction experiments, together with pharmacological approaches with aset of specific agonists and antagonists, demonstrated the presence of thethree VIP/PACAP receptor subtypes (PAC(1), VPAC(1). and VPAC(2)) with a major role for VPAC(1), acting through adenylate cyclase (AC) stimulation. Anessentially similar pattern was observed by NE differentiated cells (4 days after serum deprivation) in spite of the important morphological changes observed. However, the expression of the prostate-specific antigen (PSA) decreased in NE cells (and increased again by dihydrotestosterone, DHT, treatment). The present demonstration of the induction of NE transdifferentiation in LNCaP cells by increasing concentrations of VIP adds value to previousobservations on the role of cAMP in this process, an interesting topic in the comprehension of the molecular changes that are involved in the progression of prostate cancer to androgen independence. (C) 2001 Elsevier ScienceInc. Ail rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 10:15:38