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Titolo:
Molecular cloning and structural analysis of human sterol C5 desaturase
Autore:
Sugawara, T; Fujimoto, Y; Ishibashi, T;
Indirizzi:
Hokkaido Univ, Sch Med, Dept Biochem, Kita Ku, Sapporo, Hokkaido 0608638, Japan Hokkaido Univ Sapporo Hokkaido Japan 0608638 oro, Hokkaido 0608638, Japan
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
fascicolo: 3, volume: 1533, anno: 2001,
pagine: 277 - 284
SICI:
1388-1981(20011031)1533:3<277:MCASAO>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
REGULATORY PROTEIN STAR; 3' UNTRANSLATED REGION; LEMLI-OPITZ SYNDROME; CHOLESTEROL-METABOLISM; EXPRESSION; GENE; DEFICIENCY; PROMOTER; PATHWAY; MUTANT;
Keywords:
human sterol C5 desaturase; cholesterol; lathosterol; 25-hydroxycholesterol;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Sugawara, T Hokkaido Univ, Sch Med, Dept Biochem, Kita Ku, Kita 15,Nishi 7, Sapporo, Hokkaido 0608638, Japan Hokkaido Univ Kita 15,Nishi 7 Sapporo Hokkaido Japan 0608638 n
Citazione:
T. Sugawara et al., "Molecular cloning and structural analysis of human sterol C5 desaturase", BBA-MOL C B, 1533(3), 2001, pp. 277-284

Abstract

Sterol C5 desaturase (SC5D) converts lathosterol to 7-dehydrocholesterol in cholesterol biosynthesis. In this study, we investigated the genome structure of SC5D and transcription of the human SC5D gene to try to elucidate the mechanism by which cholesterol synthesis is regulated. The SC5D gene hada structural gene from a single copy from genome DNA that contained five exons and four introns. The human SC5D was found to be located in chromosome11q24.2-24.3 by fluorescence in situ hybridization mapping. Human SC5D mRNA transcripts, of which the major transcript was a 2 kb and the minor transcripts were 8 kb and 1.4 kb mRNA transcripts, were detected in almost all of the tissues examined. The human SC5D gene contained a GC box instead of aTATA box upstream of the transcript start sites. Human SC5D transcription started from several transcription start sites, and the first start site was located 31 bp upstream of the translation start site (ATG). The expression level of SC5D mRNA extracted from human Ever carcinoma cells decreased asthe amount of cholesterol added to the culture medium was increased. Inhibition of SC5D transcription was ascribed to the suppression of promoter activity of SC5D. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 12:06:16