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Titolo:
Autologous lymphocyte-monocyte co-culture increases NMR-visible and cytoplasmic lipids in the absence of increased markers of lymphocyte activation
Autore:
Delikatny, EJ; Pellekaan, CJV; King, NJC;
Indirizzi:
Univ Sydney, Dept Canc Med, Sydney, NSW 2006, Australia Univ Sydney Sydney NSW Australia 2006 nc Med, Sydney, NSW 2006, Australia Univ Sydney, Dept Pathol, Sydney, NSW 2006, Australia Univ Sydney Sydney NSW Australia 2006 Pathol, Sydney, NSW 2006, Australia
Titolo Testata:
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
fascicolo: 3, volume: 1533, anno: 2001,
pagine: 243 - 254
SICI:
1388-1981(20011031)1533:3<243:ALCINA>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
PLASMA-MEMBRANE TRIGLYCERIDE; BREAST CELL-LINE; MAGNETIC-RESONANCE; TETRAPHENYLPHOSPHONIUM CHLORIDE; T-LYMPHOCYTES; DROPLETS; PROLIFERATION; SIGNALS; H-1-NMR; SPECTRA;
Keywords:
lipid accumulations; neutral lipid; H-1 nuclear magnetic resonance; lymphocyte activation; NMR-visible lipid; lymphocyte-monocyte interaction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Delikatny, EJ Univ Penn, Med Ctr, Dept Radiol, Rm B1,Stellar Chance Bldg,422 Curie Blvd,Philadelphia, PA 19104 USA Univ Penn Rm B1,Stellar Chance Bldg,422 Curie Blvd Philadelphia PA USA 19104
Citazione:
E.J. Delikatny et al., "Autologous lymphocyte-monocyte co-culture increases NMR-visible and cytoplasmic lipids in the absence of increased markers of lymphocyte activation", BBA-MOL C B, 1533(3), 2001, pp. 243-254

Abstract

Alterations in nuclear magnetic resonance (NMR)-visible lipid, morphometric lipid volume fraction, distribution of subcellular lipid droplets and activation antigen expression were examined in human peripheral blood lymphocytes, activated using phorbol myristate acetate (PMA) and ionomycin or by co-culture with autologous monocytes. PMA/Ionomycin treatment caused significant time-dependent increases in mobile lipid and in oil red O-positive lipid droplets that were accompanied by lymphocyte proliferation and increases in activation antigens, CD25, CD69 and CD71. Co-culture of lymphocytes and monocytes also induced significant increases in NMR-visible lipid signals and cytoplasmic lipid droplets, but in contrast, no correspondent increases in activation antigens were observed. Strong correlations were observed between the intensity of the NMR signal and the percentage of total cells containing lipid droplets (r = 0.95) and the morphometric lymphocyte lipid volume fraction (r = 0.80), indicating that the droplets were the source of themobile lipid signal. Lipid droplets in PMA/Ionomycin-treated cells were evenly distributed throughout the population, but in co-cultures, only lymphocytes in close proximity to monocytes with lipid droplets contained oil redO-positive lipid. This data shows that the NMR-visible mobile lipid signalobserved in lymphocytes co-cultured with monocytes is not directly dependent on either proliferation or the upregulation of activation antigens, similar to the previously observed response of T cells exposed to antibodies tothe T cell receptor. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 21:40:15