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Titolo:
Hepatocyte Nuclear factors-1 alpha, -1 beta, and -3 beta expressed in the gonad of tilapia (Oreochromis mossambicus)
Autore:
Huang, WT; Gong, HY; Lin, CJF; Weng, CF; Chen, MHC; Wu, JL;
Indirizzi:
Acad Sinica, Inst Zool, Taipei 11529, Taiwan Acad Sinica Taipei Taiwan 11529 Sinica, Inst Zool, Taipei 11529, Taiwan Natl Def Med Ctr, Grad Inst Life Sci, Taipei, Taiwan Natl Def Med Ctr Taipei Taiwan Ctr, Grad Inst Life Sci, Taipei, Taiwan Natl Dong Hwa Univ, Inst Biotechnol, Hualien 97401, Taiwan Natl Dong Hwa Univ Hualien Taiwan 97401 iotechnol, Hualien 97401, Taiwan
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 4, volume: 288, anno: 2001,
pagine: 833 - 840
SICI:
0006-291X(20011109)288:4<833:HNFA-B>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR-I; TRANSCRIPTION FACTOR; MESSENGER-RNA; CDNA SEQUENCE; FACTOR 3-BETA; MOUSE EMBRYO; INSULIN; GENE; PROMOTER; HNF-3-BETA;
Keywords:
hepatocyte nuclear factor (HNF); HNF1-alpha; HNF-1 beta; HNF-3 beta; gonads; tilapia (Oreochromis mossambicus);
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Wu, JL Acad Sinica, Inst Zool, Nankang, Taipei 11529, Taiwan Acad Sinica Nankang Taipei Taiwan 11529 kang, Taipei 11529, Taiwan
Citazione:
W.T. Huang et al., "Hepatocyte Nuclear factors-1 alpha, -1 beta, and -3 beta expressed in the gonad of tilapia (Oreochromis mossambicus)", BIOC BIOP R, 288(4), 2001, pp. 833-840

Abstract

Hepatocyte nuclear factors (HN-Fs) are upstream regulators of many liver-specific genes and are involved in many cellular functions in the body, but their existence, expression, and function in gonads are still poorly understood. Here we report on the first cloning of partial cDNAs of HNF-1 alpha and -1 beta and full HNF-3 beta cDNA from a tilapia (Oreochromis mossambicus) liver cDNA library. The deduced amino acid sequence of tilapia HNF-3 betahas a 90 to 96% identity with those of other fishes (dwarf gourami, medaka, and zebrafish), 75% with mammals (human, rat, and mouse), and 82% with Xenopus. RT-PCR detected IGF-I and -II and HNF-1 alpha, -1 beta, and -3 beta in both liver and gonads and the identity of the PCR fragments was confirmed by PCR hybridization. Immunoprecipitation and Western blotting also detected all three HNF proteins in both liver and gonads. Expression of HNFs in the gonads of the tilapia suggests that multi-HNFs may form a cascade to regulate gonadal physiology in the bony fish. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 03:34:18