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Titolo:
Diagnostic DNA markers for cereal cyst nematode resistance in bread wheat
Autore:
Ogbonnaya, FC; Subrahmanyam, NC; Moullet, O; de Majnik, J; Eagles, HA; Brown, JS; Eastwood, RF; Kollmorgen, J; Appels, R; Lagudah, ES;
Indirizzi:
CSIRO Plant Ind, Canberra, ACT 2601, Australia CSIRO Plant Ind Canberra ACT Australia 2601 Canberra, ACT 2601, Australia CRC Mol Plant Breeding, Glen Osmond, SA 5064, Australia CRC Mol Plant Breeding Glen Osmond SA Australia 5064 , SA 5064, Australia
Titolo Testata:
AUSTRALIAN JOURNAL OF AGRICULTURAL RESEARCH
fascicolo: 11-12, volume: 52, anno: 2001,
pagine: 1367 - 1374
SICI:
0004-9409(2001)52:11-12<1367:DDMFCC>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
HETERODERA-AVENAE WOLL; ASSISTED SELECTION; TRITICUM-TAUSCHII; HEXAPLOID WHEAT; AEGILOPS-VENTRICOSA; QUANTITATIVE TRAITS; D-GENOME; GENE; RFLP; EFFICIENCY;
Keywords:
resistance gene; molecular markers; marker-assisted selection;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Ogbonnaya, FC Victorian Inst Dryland Agr, Dept Nat Resources & Environm, Natimuk Rd,PB 260, Horsham, Vic 3401, Australia Victorian Inst Dryland Agr Natimuk Rd,PB 260 Horsham Vic Australia 3401
Citazione:
F.C. Ogbonnaya et al., "Diagnostic DNA markers for cereal cyst nematode resistance in bread wheat", AUST J AGR, 52(11-12), 2001, pp. 1367-1374

Abstract

The development of cultivars resistant to cereal cyst nematode (CCN) is a primary objective in wheat breeding in the southern wheatbelt of Australia. Nine CCN resistance genes have been identified in wheat and its relatives,some of which confer resistance to the Australian pathotype of CCN (Ha13). Cultivars released in Australia with CCN resistance carry either the Cre1 or CreF gene, with the Cre3 gene present in advanced breeding lines. The biological assay for CCN resistance screening in wheat is time-consuming, notreliable on a single-plant basis, and prone to inconsistencies, thus reducing the efficiency of selection amongst breeding lines. Using gene sequences initially isolated from the Cre3 locus, a DNA-based marker selection system was developed and applied to unambiguously identify wheat lines carryingresistance alleles at the Cre1 and/or Cre3 loci in breeding populations derived from diverse genetic backgrounds. Homologues of sequences from the Cre3 locus, located elsewhere in the wheat genome, can also be used to selectwheat lines with a newly identified CCN resistance gene (Cre6) introgressed from Aegilops ventricosa. Application of these markers has become an integral part of the southern Australian breeding programs.

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Documento generato il 29/11/20 alle ore 18:09:17