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Titolo:
Predominance of G3B and G14 equine group A rotaviruses of a single VP4 serotype in Japan
Autore:
Tsunemitsu, H; Imagawa, H; Togo, M; Shouji, T; Kawashima, K; Horino, R; Imai, K; Nishimori, T; Takagi, M; Higuchi, T;
Indirizzi:
Natl Inst Anim Hlth, Shichinohe Res Unit, Shichinohe, Aomori 0392596, Japan Natl Inst Anim Hlth Shichinohe Aomori Japan 0392596 Aomori 0392596, Japan Ishikari Livestock Hyg Ctr, Sapporo, Hokkaido, Japan Ishikari Livestock Hyg Ctr Sapporo Hokkaido Japan pporo, Hokkaido, Japan Natl Inst Anim Hlth, Hokkaido Res Stn, Sapporo, Hokkaido, Japan Natl Inst Anim Hlth Sapporo Hokkaido Japan Stn, Sapporo, Hokkaido, Japan Kobe Univ, Fac Agr, Dept Microbiol & Immunol, Kobe, Hyogo 657, Japan Kobe Univ Kobe Hyogo Japan 657 icrobiol & Immunol, Kobe, Hyogo 657, Japan
Titolo Testata:
ARCHIVES OF VIROLOGY
fascicolo: 10, volume: 146, anno: 2001,
pagine: 1949 - 1962
SICI:
0304-8608(2001)146:10<1949:POGAGE>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; CAPSID PROTEIN VP4; GENOMIC CHARACTERIZATION; MONOCLONAL-ANTIBODIES; PORCINE ROTAVIRUS; SEQUENCE-ANALYSIS; BOVINE ROTAVIRUSES; GLYCOPROTEIN VP7; P-SEROTYPES; IDENTIFICATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Tsunemitsu, H Natl Inst Anim Hlth, Shichinohe Res Unit, Shichinohe, Aomori0392596, Japan Natl Inst Anim Hlth Shichinohe Aomori Japan 0392596 , Japan
Citazione:
H. Tsunemitsu et al., "Predominance of G3B and G14 equine group A rotaviruses of a single VP4 serotype in Japan", ARCH VIROL, 146(10), 2001, pp. 1949-1962

Abstract

A total of 65 equine group A rotaviruses (GAR) isolated from diarrheal foals at 48 farms in Hokkaido, Japan, between 1996 (29 isolates) and 1997 (36 isolates) were characterized for their VP7 and VP4 serotypes by PCR, nucleotide sequencing, and virus neutralization (VN) tests. By PCR VP7 typing, all isolates were classified as G3 or G14, and the predominant serotype in each year was G3 (86%) in 1996 and G14 (53%) in 1997. VN tests with these 20 isolates randomly selected confirmed the specificity of PCR on the bases ofcomplete agreement of the results in these methods (9 G3 and 11 G14), and revealed that all 9 G3 isolates were subtype G3B. There were five differingamino acid residues in three VP7 antigenic regions between subtypes G3A and G3B. Antiserum to a baculovirus recombinant that expressed P[12] VP4 neutralized all isolates and P[12] reference strains. These results suggest that genotype P[12] GAR belong to a single VP4 serotype, and that one VP4 and two VP7 serotypes (G3B and G14) of GAR were predominant in the equine population in Japan.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 23:55:17