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Titolo:
Genomic heterogeneity in Pea seed-borne mosaic virus isolates from Pakistan, the centre of diversity of the host species, Pisum sativum
Autore:
Ali, A; Randles, JW;
Indirizzi:
Univ Adelaide, Dept Appl & Mol Ecol, Glen Osmond, SA 5064, Australia Univ Adelaide Glen Osmond SA Australia 5064 en Osmond, SA 5064, Australia
Titolo Testata:
ARCHIVES OF VIROLOGY
fascicolo: 10, volume: 146, anno: 2001,
pagine: 1855 - 1870
SICI:
0304-8608(2001)146:10<1855:GHIPSM>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENETIC-VARIABILITY; NUCLEOTIDE-SEQUENCE; POINT MUTATIONS; POTYVIRUS; RNA; PROTEIN; AMPLIFICATION; PATHOTYPES; ASSAY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Randles, JW Univ Adelaide, Dept Appl & Mol Ecol, Waite Campus, Glen Osmond, SA 5064, Australia Univ Adelaide Waite Campus Glen Osmond SA Australia 5064 ralia
Citazione:
A. Ali e J.W. Randles, "Genomic heterogeneity in Pea seed-borne mosaic virus isolates from Pakistan, the centre of diversity of the host species, Pisum sativum", ARCH VIROL, 146(10), 2001, pp. 1855-1870

Abstract

A range of isolates of Pea seed-borne mosaic virus (PSbMV) was compared inthe segments of the genome representing the partial NIb/CP/UTR and the partial P1-Pro/HC-Pro coding regions. Nucleotide and amino acid sequences, anda phylogenetic analysis of the CP region, divided isolates with available sequence information into two groups, one representing pathotype 4, the other pathotype 1. The pathotype 1 group showed greater diversity than the pathotype 4 group. A comparison of 14 isolates, S6 (a pathotype 4 isolate), US(a pathotype 1 isolate) and 12 isolates from Pakistan, by ribonuclease protection assay (RPA) using cRNA transcripts of the cloned partial NIb/CP/UTRregions of the S6, US and Pakistani isolate PK9 placed them into three distinct phylogenetic groups. RPA with a partial P1-Pro/HC-Pro cRNA probe identified a greater level of variation which was too high to be used for generating an overall phylogeny. Thus, RPA identified greater, molecular diversity in PSbMV than described hitherto. We conclude that, in addition to the pathotypes 1 and 4 typified by US and S6 respectively, isolates of PSbMV from Pakistan include previously unrecognised molecular variants, and this accords with our previous recognition of new pathotypes from Pakistan.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 13:11:55