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Titolo:
Anti-U1RNP antibodies in patients with localized scleroderma
Autore:
Yamane, K; Ihn, H; Kubo, M; Kuwana, M; Asano, Y; Yazawa, N; Tamaki, K;
Indirizzi:
Univ Tokyo, Fac Med, Dept Dermatol, Bunkyo Ku, Tokyo 1138655, Japan Univ Tokyo Tokyo Japan 1138655 Dermatol, Bunkyo Ku, Tokyo 1138655, Japan Keio Univ, Sch Med, Inst Adv Med Res, Div Cellular Signaling, Tokyo, JapanKeio Univ Tokyo Japan Adv Med Res, Div Cellular Signaling, Tokyo, Japan
Titolo Testata:
ARCHIVES OF DERMATOLOGICAL RESEARCH
fascicolo: 9, volume: 293, anno: 2001,
pagine: 455 - 459
SICI:
0340-3696(200109)293:9<455:AAIPWL>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
CONNECTIVE-TISSUE DISEASE; SMALL NUCLEAR RIBONUCLEOPROTEINS; SYSTEMIC LUPUS-ERYTHEMATOSUS; MESSENGER-RNA PRECURSORS; ANTINUCLEAR ANTIBODIES; ANTIHISTONE ANTIBODIES; ADHESION MOLECULE-1; ANTIGEN-SPECIFICITY; LINEAR SCLERODERMA; SERUM LEVELS;
Keywords:
autoantibody; immunoprecipitation; immunoblotting;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Ihn, H Univ Tokyo, Fac Med, Dept Dermatol, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1138655,Japan Univ Tokyo 7-3-1 Hongo Tokyo Japan 1138655 go, Tokyo 1138655,Japan
Citazione:
K. Yamane et al., "Anti-U1RNP antibodies in patients with localized scleroderma", ARCH DERM R, 293(9), 2001, pp. 455-459

Abstract

Antibodies to U1 ribonucleoproteins (RNP) have been detected in serum frompatients with various autoimmune diseases. However, the presence of anti-U1RNP antibodies in patients with localized scleroderma has not been reported. In this study, we examined the frequency of anti-U1RNP antibodies using immunoprecipitation of U small nuclear RNAs and determined the antigen specificity by immunoblotting. Of 70 serum samples from patients with localizedscleroderma, 2 (3%) immunoprecipitated U1 small nuclear RNA. Indirect immunofluorescence using HEp-2 cells as substrate showed coarse speckled nuclear fluorescence without nucleolar staining in both of the samples positive for anti-U1RNP antibodies. In addition, the presence of anti-U1RNP antibodies in each serum sample was confirmed by immunodiffusion against HeLa cell extracts. Immunoblotting analysis showed anti-70 kDa antibodies in each serum sample. This reaction against 70 kDa protein in the patients with localized scleroderma was analogous to that in patients with systemic sclerosis ormixed connective tissue disease. Both patients with positive serum were diagnosed as having linear scleroderma, but neither had evidence of Raynaud'sphenomenon or sclerodactyly. These results indicate that the presence of anti-U1RNP antibodies is one of the serological abnormalities in localized scleroderma, and that the mechanism of induction of anti-U1RNP antibodies inpatients with localized scleroderma might be similar to that in patients with systemic sclerosis and mixed connective tissue disease.

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Documento generato il 20/01/21 alle ore 01:41:55