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Titolo:
Three-dimensional imaging of hormone-secreting cells and their microvesselenvironment in estrogen-induced prolactinoma of the rat pituitary gland byconfocal laser scanning microscopy
Autore:
Itoh, J; Kawai, T; Serizawa, A; Yamamoto, Y; Ogawa, K; Matsuno, A; Watanabe, K; Osamura, RY;
Indirizzi:
Tokai Univ, Sch Med, Labs Struct & Funct Res, Isehara, Kanagawa 2591193, Japan Tokai Univ Isehara Kanagawa Japan 2591193 sehara, Kanagawa 2591193, Japan Tokai Univ, Sch Med, Div Diagnost Pathol, Isehara, Kanagawa 2591193, JapanTokai Univ Isehara Kanagawa Japan 2591193 sehara, Kanagawa 2591193, Japan Tokai Univ, Sch Med, Dept Pathol, Isehara, Kanagawa 2591193, Japan Tokai Univ Isehara Kanagawa Japan 2591193 sehara, Kanagawa 2591193, Japan NKK Hosp, Dept Diagnost Radiol, Kawasaki, Kanagawa, Japan NKK Hosp Kawasaki Kanagawa Japan gnost Radiol, Kawasaki, Kanagawa, Japan NKK Hosp, Pathol Div, Clin Lab, Kawasaki, Kanagawa, Japan NKK Hosp Kawasaki Kanagawa Japan iv, Clin Lab, Kawasaki, Kanagawa, Japan Teikyo Univ, Ichihara Hosp, Dept Neurosurg, Chiba, Japan Teikyo Univ Chiba Japan iv, Ichihara Hosp, Dept Neurosurg, Chiba, Japan Aobagakuen Jr Coll, Tokyo, Japan Aobagakuen Jr Coll Tokyo JapanAobagakuen Jr Coll, Tokyo, Japan
Titolo Testata:
APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY
fascicolo: 4, volume: 9, anno: 2001,
pagine: 364 - 370
SICI:
1062-3345(200112)9:4<364:TIOHCA>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANTIHEMOPHILIC FACTOR ANTIGEN; HUMAN-ENDOTHELIAL CELLS; 3-DIMENSIONAL RECONSTRUCTION; ULTRASTRUCTURAL-LOCALIZATION; ELECTRON-MICROSCOPE; BROMOCRIPTINE; MICROCIRCULATION; VASCULARIZATION; VISUALIZATION; ANGIOGENESIS;
Keywords:
confocal laser scanning microscopy; estrogen-induced prolactinoma; hormone-secreting cell; microvessel environment; three-dimensional imaging;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Itoh, J Tokai Univ, Sch Med, Labs Struct & Funct Res, Isehara, Kanagawa 2591193, Japan Tokai Univ Isehara Kanagawa Japan 2591193 Kanagawa 2591193, Japan
Citazione:
J. Itoh et al., "Three-dimensional imaging of hormone-secreting cells and their microvesselenvironment in estrogen-induced prolactinoma of the rat pituitary gland byconfocal laser scanning microscopy", APPL IMMUNO, 9(4), 2001, pp. 364-370

Abstract

This study focused on the three-dimensional imaging of hormone-secreting cells and their microvascular environment in estrogen-induced prolactinoma of the rat pituitary gland. Adult female Wistar-Imamichi rats were injected with estradiol dipropionate and killed 7 weeks later. Some rats given estrogen for 7 weeks also were injected with bromocriptine before killing. To obtain a detailed three-dimensional image of microvessels, dialyzed fluorescein isothiocyanate (FITC)-conjugated gelatin was injected into the left ventricle of the rat heart. After the perfusion, the pituitary glands were resected and subjected to immunohistochemistry (IHC). To evaluate the effects of estro-en and bromocriptine, IHC was performed with antibodies against prolactin (PRL), adrenocorticotropic hormone (ACTH), and growth hormone (GH). With the combination, microvessels and cells containing PRL, ACTH, and GH could be clearly identified by confocal laser scanning microscopy (CLSM). The PRL cells increased in number and became hypertrophic after prolonged exposure to estrogen. With bromocriptine administration after estrogen treatment, however, PRL cells decreased in number and became atrophic. The currentstudy revealed that estrogen and bromocriptine had significant effects on PRL secretion and the microvascular environment. Therefore, this technique (FITC injection and IHC) with CLSM is suitable for the three-dimensional imaging of hormone-secreting mechanisms under various conditions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 00:47:06