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Titolo:
Cytologic and DNA-cytometric early diagnosis of oral cancer
Autore:
Remmerbach, TW; Weidenbach, H; Pomjanski, N; Knops, K; Mathes, S; Hemprich, A; Bocking, A;
Indirizzi:
Univ Leipzig, Dept Oral Maxillofacial & Plast Surg, D-04103 Leipzig, Germany Univ Leipzig Leipzig Germany D-04103 last Surg, D-04103 Leipzig, Germany Univ Leipzig, Inst Pathol, D-04103 Leipzig, Germany Univ Leipzig LeipzigGermany D-04103 st Pathol, D-04103 Leipzig, Germany Univ Dusseldorf, Inst Cytopathol, D-40225 Dusseldorf, Germany Univ Dusseldorf Dusseldorf Germany D-40225 , D-40225 Dusseldorf, Germany
Titolo Testata:
ANALYTICAL CELLULAR PATHOLOGY
fascicolo: 4, volume: 22, anno: 2001,
pagine: 211 - 221
SICI:
0921-8912(2001)22:4<211:CADEDO>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMAGE-CYTOMETRY; EPITHELIAL DYSPLASIA; NEOPLASTIC-CELLS; LESIONS; CARCINOMAS; ANEUPLOIDY; IDENTIFICATION; CYTOBRUSH; CAVITY; AID;
Keywords:
oral cancer; oral exfoliative cytology; DNA-aneuploidy; DNA-image cytometry; cancer screening; diagnostic accuracy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Remmerbach, TW Univ Leipzig, Dept Oral Maxillofacial & Plast Surg, Nurnberger Str 57, D-04103 Leipzig, Germany Univ Leipzig Nurnberger Str 57 Leipzig Germany D-04103 any
Citazione:
T.W. Remmerbach et al., "Cytologic and DNA-cytometric early diagnosis of oral cancer", ANAL CELL P, 22(4), 2001, pp. 211-221

Abstract

Objective. The aim of this prospective study was to report on the diagnostic accuracy of conventional oral exfoliative cytology taken from white-spotted, ulcerated or other suspicious oral lesions in our clinic. In addition we checked DNA-image cytometry as an adjuvant diagnostic tool. Our hypothesis is that DNA-aneuploidy is a sensitive and specific marker for the early identification of tumor cells in oral brushings. Study design. 251 cytological diagnoses obtained from exfoliative smears of 181 patients from macroscopically suspicious lesions of the oral mucosa and from clinically seemingly benign oral lesions which were exisiced for establishing histological diagnoses were compared with histological and/or clinical follow-ups of the respective patients. Additionally nuclear DNA-contents were measured after Feulgen restaining using a TV image analysis system. Results. Sensitivity of our cytological diagnosis on oral smears for the detection of cancer cells was 94.6%, specificity 99.5%, positive predictive value 98.1% and negative predictive value 98.5%. DNA-aneuploidy was assumed if abnormal DNA-stemlines or cells with DNA-content greater 9c were observed. On this basis the prevalence of DNA-aneuploidy in smears of oral squamous cell carcinomas in situ or invasive carcinomas was 96.4%. Sensitivity of DNA-aneuploidy in oral smears for the detection of cancer cells was 96.4%, specificity 100%, positive predictive value 100% and negative 99.0%. The combination of both techniques increased the sensivity to 98.2%, specificity to 100%, positive predictive value to 100% and negative to 99.5%. Conclusions. Brush cytology of all visible oral lesions, if they are clinically considered as suspicious forcancer, are an easily practicable, cheap. non-invasive, painless. safe andaccurate screening method for detection of oral precancerous lesions, carcinoma in situ or invasive squamous cell carcinoma in all stages. We conclude that DNA-image cytometry is a very sensitive, highly specific and objective adjuvant tool for the early identification of neoplastic epithelial cells in oral smears.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 23:58:14