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Titolo:
Regional specificity of ethanol and NMDA action in brain revealed with FOS-like immunohistochemistry and differential routes of drug administration
Autore:
Knapp, DJ; Braun, CJ; Duncan, GE; Qian, Y; Fernandes, A; Crews, FT; Breese, GR;
Indirizzi:
Univ N Carolina, Bowles Ctr Alcohol Studies, Chapel Hill, NC 27599 USA Univ N Carolina Chapel Hill NC USA 27599 udies, Chapel Hill, NC 27599 USA Univ N Carolina, Ctr Neurosci, Chapel Hill, NC 27599 USA Univ N Carolina Chapel Hill NC USA 27599 rosci, Chapel Hill, NC 27599 USA Univ N Carolina, Dept Psychiat, Chapel Hill, NC 27599 USA Univ N CarolinaChapel Hill NC USA 27599 chiat, Chapel Hill, NC 27599 USA Univ N Carolina, Dept Pharmacol, Chapel Hill, NC 27599 USA Univ N Carolina Chapel Hill NC USA 27599 macol, Chapel Hill, NC 27599 USA Univ N Carolina, Dept Anesthesiol, Chapel Hill, NC 27599 USA Univ N Carolina Chapel Hill NC USA 27599 esiol, Chapel Hill, NC 27599 USA
Titolo Testata:
ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH
fascicolo: 11, volume: 25, anno: 2001,
pagine: 1662 - 1672
SICI:
0145-6008(200111)25:11<1662:RSOEAN>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
PITUITARY-ADRENAL AXIS; EVOKED ELECTROPHYSIOLOGICAL ACTIVITY; PUPILLARY LIGHT REFLEX; D-ASPARTATE RESPONSES; INBRED MOUSE STRAINS; IMMEDIATE-EARLY GENE; C-FOS; RAT-BRAIN; MESSENGER-RNA; PARAVENTRICULAR NUCLEUS;
Keywords:
ethanol; NMDA; Fos; route of administration; brain;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
72
Recensione:
Indirizzi per estratti:
Indirizzo: Knapp, DJ Univ N Carolina, Bowles Ctr Alcohol Studies, CB7178, Chapel Hill, NC 27599USA Univ N Carolina CB7178 Chapel Hill NC USA 27599 ill, NC 27599USA
Citazione:
D.J. Knapp et al., "Regional specificity of ethanol and NMDA action in brain revealed with FOS-like immunohistochemistry and differential routes of drug administration", ALC CLIN EX, 25(11), 2001, pp. 1662-1672

Abstract

Background: Inhibition of NMDA receptor function in brain is believed to be an important action of ethanol (EtOH). To investigate EtOH inhibition of NMDA receptor responses in vivo, the interaction of these agents in brain after different routes of administration were investigated by using transcription factor Fos protein expression to follow NMDA receptor activation and EtOH inhibition of this response. Methods: The induction of Fos-like immunoreactivity (Fos-LI) in 38 regionsof the rat brain was measured 2 hr after treatment with NMDA, EtOH, or both. To determine the relative contribution of abdominal drug effects on Fos induction, rats received either intraperitoneal (ip) or intragastric (ig) EtOH and ip or intravenous (iv) NMDA. Rats received EtOH (2.5 g/kg ip or 4 g/kg ig) or vehicle 15 min before NMDA (125 mg/kg ip or 60 mg/kg iv) or vehicle. Results: For the 38 forebrain regions examined, ip and iv NMDA significantly induced Fos-LI in 13 and 32 regions, respectively. These effects occurred without elicitation of tonic-clonic seizure activity and were strong after iv NMDA in the frontal, prefrontal, and cingulate cortices, supraoptic nucleus, anterior lateral septum, and dentate gyrus. For EtOH, prominent Fos-LI induction was found in the central amygdala, dorsolateral bed nucleus ofthe stria terminalis, Edinger-Westphal nucleus, and paraventricular hypothalamus. Despite ip and ig EtOH induction of Fos-LI in these regions, the major effect of EtOH was to block NMDA-induced Fos-LI in 8 of 13 (ip) and 27 of 32 (ig) of the NMDA-positive regions, respectively, including retrosplenial, cingulate, and medial prefrontal cortices, central amygdala, and taenia tecta. Conclusions: These results provide new evidence for the regionally specific functional interactions of EtOH on NMDA receptors in vivo. Moreover, these results support efforts to identify brain region-specific targets for EtOH and EtOH-induced changes in gene expression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 19:56:32