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Titolo:
Vaccination of mice with live recombinant Salmonella typhimurium aroA against H-pylori: parameters associated with prophylactic and therapeutic vaccine efficacy
Autore:
Koesling, J; Lucas, B; Develioglou, L; Aebischer, T; Meyer, TF;
Indirizzi:
Max Planck Inst Infect Biol, Dept Mol Biol, D-10117 Berlin, Germany Max Planck Inst Infect Biol Berlin Germany D-10117 10117 Berlin, Germany
Titolo Testata:
VACCINE
fascicolo: 3-4, volume: 20, anno: 2001,
pagine: 413 - 420
SICI:
0264-410X(20011112)20:3-4<413:VOMWLR>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
HELICOBACTER-PYLORI; IMMUNE-RESPONSE; BALB/C MICE; ORAL IMMUNIZATION; HETEROLOGOUS ANTIGEN; BACTERIAL CLEARANCE; DIFFERENT PROMOTERS; ANTIBODY-RESPONSES; FELIS INFECTION; GENETIC-CONTROL;
Keywords:
spleen colonization; gastric mucosa; humoral immune response;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Meyer, TF Max Planck Inst Infect Biol, Dept Mol Biol, Schumannstr 21-22, D-10117 Berlin, Germany Max Planck Inst Infect Biol Schumannstr 21-22 Berlin Germany D-10117
Citazione:
J. Koesling et al., "Vaccination of mice with live recombinant Salmonella typhimurium aroA against H-pylori: parameters associated with prophylactic and therapeutic vaccine efficacy", VACCINE, 20(3-4), 2001, pp. 413-420

Abstract

Previously we described a recombinant attenuated Salmonella typhimurium aroA strain (SL3261 [pYZ97]) with constitutive expression of plasmid encoded Helicobacter pylori urease subunits A and B (UreAB). Single dose oral vaccination effectively induced prophylactic immunity against bacterial challenge in BALB/c mice. Here we successfully extended this approach to several mouse strains with allelic differences in NRAMP-1 and H-2 genes. The respective host determinants are known to influence the immune response against S. typhimurium. A comparative analysis of the vaccine efficacy in C57BL/6 and BALB/c mice showed that the live vaccine confers long lasting immunity in both strains (> 18 weeks). In C57BL/6 mice, protection was still observed 54weeks while not all vaccinated BALB/c were immune when challenged after this time. BALB/c mice also needed higher doses of SL3261 [pYZ97] for full protection. We also demonstrate a therapeutic potential of SL3261 [pYZ97] in H. pylori infected BALB/c and C57BL/6 mice. Urease- and carrier- specific ser-um antibody responses as well as the level of colonization by the Salmonella were analyzed in both mouse strains after immunization with low (4 x 10(7) CFU) or high (I x 10(9) CFU) vaccine doses. The results are discussed in the context of inoculum size and the mode of antigen supply required foreffective vaccination with recombinant Salmonella. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 06:56:48